Anti-8-Nitroguanine Antibody, clone NO2-52

32160702

8-Nitroguanosine, a highly reactive redox-active nucleic acid derivative, is formed by the action of peroxynitrite. It is a nitrated base of DNA and RNA formed as a result of chronic inflammation. It has been well established that large amount of nitric oxide molecules and superoxide anion, generated as a result of inflammatory response, can cause nitration of guanosine. Such chemically modified nucleotides increase the incidence of mutation during DNA replication. 8-nitroguanosine is considered as a marker of DNA damage related to mutation and cancer. Nitrated guanine nucleosides and nucleotides in the nucleotide pool are shown to contribute to oxidative stress via production of superoxide mediated by various reductases and may disturb or modulate directly various important proteins and enzymes, such as G-proteins and cGMP-dependent enzymes. Clone NO2-52 is a highly specific monoclonal antibody that recognizes 8-nitroguanine and 8-nitroguanosine. However, it does not cross-react with normal nucleotide bases. (Ref.: Ohshima, H., et al. (2006). Antioxid. Redox Signal 8(5-6); 1033-45; Sawa, T., et al. (2007). Nat. Chem. Biol. 3(11); 727-35).

Clone NO2-52 specifically detects 8-nitroguanine.

BSA conjugated 8-nitroguanosine.

ELISA Analysis: A representative lot detected 8-Nitroguanine in ELISA applications (Sawa, T., et. al. (2007). Nat Chem Biol. 3(11):727-35; Yoshitake, J., et. al. (2004). J Virol. 78(16):8709-19).

Immunocytochemistry Analysis: A representative lot detected 8-Nitroguanine in Immunocytochemistry applications (Ihara, H., et. al. (2011). Nitric Oxide. 25(2):169-75; Sawa, T., et. al. (2007). Nat Chem Biol. 3(11):727-35; Yoshitake, J., et. al. (2004). J Virol. 78(16):8709-19).

Anti-8-Nitroguanine, clone NO2-52, Cat. No. MABN2411, is a highly specific mouse monoclonal antibody that targets 8-Nitroguanine and has been tested for use in ELISA, Immunocytochemistry, and Western Blotting.

Evaluated by Western Blottingwith Nitroguanine-conjugated BSA (NG-BSA).

Western Blotting Analysis: 1 µg/mL of this antibody detected 8-Nitroguanine in Nitroguanine-conjugated BSA (NG-BSA). It does not react with BSA alone.

Purified by ion-exchange chromatography
Format: Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified antibody

primary antibodies

NO2-52, monoclonal

mouse, rat

ELISA: suitable
immunocytochemistry: suitable
western blot: suitable

IgG1κ

ambient

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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