Anti-5-Methylcytosine Mouse mAb (162 33 D3)

100

mouse

affinity isolated antibody

primary antibodies

162 33 D3, monoclonal

liquid

preservative

all

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG1

wet ice

−20℃

50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Immunoaffinity purified mouse monoclonal antibody. Recognizes 5-methylcytosine.
This Anti-5-Methylcytosine Mouse mAb (162 33 D3) is validated for use in ELISA, FC, Frozen Sections, IF, Paraffin Sections, Radioimmunoassay, Southwestern Blot for the detection of 5-Methylcytosine.
Recognizes 5-methylcytosine in methylated DNA or RNA in NIH3T3 cells.

Specific
• Affinity purified mouse monoclonal antibody - Detects methylated DNA from a broad range of species. Several Applications
• Flow Cytometry
• Frozen Sections
• Immunoblotting
• Immunofluorescence
• Paraffin Sections

Reliable
• Generates reproducible results.
• Clone 162 33 D3 has been used in over 50 publications.

5-methylcytosine conjugated to ovalbumin

ELISA (0.1 g/ml)
Flow Cytometry (1 g/ml)
Frozen Sections (5-10 g/ml)
Immunoblotting (5 g/ml)
Immunofluorescence (1 g/ml)
Paraffin Sections (5-10 g/ml)
Radioimmunoassay (0.1 g/ml)
Southwestern Blot (5 g/ml)

Toxicity: Standard Handling (A)

In PBS, pH 7.4.

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
NIH3T3 cells

Clone 162 33 D3 is useful for the quantitative and qualitative detection of methylated DNA or RNA in a variety of samples and applications. It has been used in over 40 publications. Antibody should be titrated for optimal results in an individual systems.
Ehrlich, M. 2002. Oncogene21, 5400. (Review)
Widschwendter, M. and Jones, P.A. 2002. Oncogene21, 5462. (Review)
Barton, S.C., et al. 2001. Hum. Mol. Genet.10, 2983.
Piyathilake, C.J., et al. 2001. Hum. Pathol.32, 856.
Taddei, A., et al. 2001. Nat. Cell Biol.3, 114.
Piyathilake, C.J., et al. 2000. Biotech. Histochem.75, 251.
De Capoa, A., et al. 1999. FASEB J.13, 89.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

11 - Combustible Solids

WGK 1