Immobilon^®-FL PVDF Membrane

32031602

NB.22

The Immobilon^®-FL transfer membrane is hydrophobic polyvinylidene fluoride (PVDF) microporous membrane used for the transfer of proteins from a variety of gel matrices. This transfer membrane offers a uniformly controlled pore structure with a high binding capacity for biomolecules. The membrane displays very low autofluorescence across a wide range of excitation/emission wavelengths. The Immobilon^®-FL membrane has a nominal pore size of 0.45 μm and offers optimal blotting for proteins with molecular weights greater than 20,000 Daltons.

Immobilon^®-FL membrane has been used in western blotting. It is also suitable for use in standard chemiluminescent or chromogenic detection.

Immobilon^®-FL Membrane:

• The first transfer membrane designed for fluorescence applications Extremely low background improves the sensitivity of all fluorescence detection protocols
• Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
• compatible with standard blocking agents and buffers
• Ideal for multiplexing and chemifluorescence detections

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

PVDF membrane
plain filter
white filter

400

hydrophobic

Immobilon^®

western blot: suitable

10 cm × 10 cm

0.45 μm pore size

155 μg/cm² adsorption capacity (insulin)
205 μg/cm² adsorption capacity (BSA)
300 μg/cm² adsorption capacity (goat IgG)

for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red

chemiluminescent
colorimetric
fluorometric

ambient

11 - Combustible Solids

WGK 3