DAPI，二盐酸盐-CAS 28718-90-3-Calbiochem

C₁₆H₁₅N₅ · 2HCl

350.25

MFCD00012681

Fluorescent dye specific for DNA. Binds preferentially to DNA rich in adenine and thymidine (excitation max. = 359 nm, emission max. = 461 nm). Useful for microscopic analysis of nuclei and nuclear DNA in apoptotic cells. Can also be used to detect mycoplasma. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.
Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H₂O (Cat. No. 508741).

Product does not compete with ATP.
Cell permeable: no
Primary Target
Binds preferentially to DNA rich in adenine and thymine
Reversible: yes

Toxicity: Standard Handling (A)

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.

Mathieu, C., et al. 1995. Exp. Cell Res.216, 13.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.
Hotz, M.A., et al. 1994. Cytometry15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.
Miller, T., et al. 1993. Biotechniques15, 1042.
Gray, J.W., et al. 1982. Science238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods38, 315.
Russels, W.C., et al. 1975. Nature253, 461.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥90% (HPLC)

solid

Calbiochem^®

OK to freeze
desiccated (hygroscopic)
protect from light

yellow

water: 5 mg/mL
ethanol: soluble
methanol: soluble

λ_ex ~359 nm
λ_em ~461 nm

ambient

−20℃

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

FPNZBYLXNYPRLR-UHFFFAOYSA-N

10 mg in Plastic ampoule

GHS07

Warning

H315 - H317 - H335

P261 - P264 - P271 - P272 - P280 - P302 + P352

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

Respiratory system

11 - Combustible Solids

WGK 3