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Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2
产品别名
Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2
mitogen-activated protein kinase 14, cytokine suppressive anti-inflammatory drug binding protein, stress-activated protein kinase 2A, Cytokine suppressive anti-inflammatory drug-binding protein, CSAID-binding protein, MAP kinase MXI2, MAX-interacting pro
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | The p38 family refers to the SAPK2/3 Kinases (Stess-Activated Protein Kinases), a subfamily of JNK/SAPK family. p38/SAPK Kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. SAPKs have been implicated in many pathological conditions, including inflammation, cancer and neurodegenerative diseases. As with the other MAPK family members (ERK, JNK, p38MAPK, and ERK5), p38 is activated through phosphorylation on both threonine and tyrosine residues at the Thr-X-Tyr dual phosphorylation motif, where X is Gly for p38. Once phosphorylated and active, it then goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. Some of their main targets of p38 are the kinase MAPKAPK2 and the transcription factor ATF2 that are activated via their phosphorylation. |
| Specificity【特异性】 | This antibody recognizes p38 when phosphorylated at Thr180 and Tyr182. |
| Immunogen【免疫原】 | Epitope: Phosphorylated Thr180 and Tyr182 Linear peptide corresponding to human p38 phosphorylated at Thr180 and Tyr182. |
| Application【应用】 | Research Sub Category MAP Kinases Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected p38 in Anisomycin treated NIH/3T3 cells. Research Category Signaling Detect phospho-p38 (Thr180/Tyr182) using this Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2 validated for use in WB & IC. |
| Quality【质量】 | Evaluated by Western Blot in Anisomycin treated and untreated HeLa cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected p38 on 10 µg of Anisomycin treated and untreated HeLa cell lysate. |
| Physical form【外形】 | Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. Format: Purified Protein G Purified |
| Analysis Note【分析说明】 | Control Anisomycin treated and untreated HeLa cell lysate |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | 6E5.2, monoclonal |
| species reactivity | mouse, human |
| technique(s) | immunocytochemistry: suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_001306.1 |
| UniProt accession no.【UniProt登记号】 | Q16539 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... MAPK14(1432) |
产品说明
| Target description【目标描述】 | 41 kDa observed |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
