Anti-Integrin α4 Antibody, cytoplasmic on CT

32160702

NA.41

Integrin alpha 4. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine-labeled lymphoid cells. No cross-reactivity to alpha 1, alpha 2, alpha 3, alpha 5, alpha 6 or alpha V. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and it is thus intracellular.

Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human alpha 4 integrin subunit.
Epitope: cytoplasmic domain, C-terminus

Research Category
Cell Structure
ELISA/RIA: 1:1000.

Western blot: 1:1000, non-reduced conditions; blocking should be with 1-3% BSA. Recognizes a 140 kDa human alpha 4 band under non-reducing conditions.

Immunohistochemistry: 1:500

Immunoprecipitation: 5 μL of antibody is sufficient to precipitate alpha4 from 5x106 cells.

Optimal working dilutions must be determined by end user.
Research Sub Category
Integrins
Anti-Integrin α4 Antibody, cytoplasmic on C-terminus is an antibody against Integrin α4 for use in ELISA, IP, RIA, WB, IH.

Replaces: 04-1131

Liquid rabbit antiserum with 0.05% sodium azide.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

serum

primary antibodies

polyclonal

chicken, rat, pig, human, mouse, hamster

Chemicon^®

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable

NM_000885.4

P13612

wet ice

Maintain at -20ºC in undiluted aliquots for up to 12 months upon date of receipt. Avoid repeated freeze/thaw cycles.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids