Melittin-CAS 37231-28-0-Calbiochem

C₁₃₁H₂₂₉N₃₉O₃₁

2846.46

A 26-residue polypeptide from bee venom that binds calmodulin in a Ca²⁺-dependent manner. A stimulator of G_iα and G₁₁α activity that is reported to inhibit adenylate cyclase activity in synaptic membranes. Activates phospholipase A₂ and inhibits protein kinase C (IC₅₀ = 5-7 µM) by binding to the catalytic domain in a Mg²⁺-ATP sensitive manner. Has also been used for affinity purification of several Ca²⁺-binding proteins.
A 26-residue polypeptide from bee venom that binds calmodulin in a Ca²⁺-dependent manner, activates phospholipase A₂, and inhibits protein kinase C (IC₅₀ = 5-7 µM) by binding to the catalytic domain in a Mg²⁺-ATP sensitive manner. A stimulator of G_iα and G₁₁α activity that is reported to inhibit adenylate cyclase activity in synaptic membranes. Has also been used for affinity purification of several Ca²⁺-binding proteins. Activates G_iα-1 and G₁₁α activities and inhibits G_s activity.

Product does not compete with ATP.
Reversible: no
Target IC₅₀: 5-7 µM 5-7 µM for activating phospholipase A2 and inhibits protein kinase C
Cell permeable: no

Toxicity: Standard Handling (A)

H-Gly-Ile-Gly-Ala-Val-Leu-Lys-Val-Leu-Thr-Thr-Gly-Leu-Pro-Ala-Leu-Ile-Ser-Trp-Ile-Lys-Arg-Lys-Arg-Gln-Gln-NH₂

Fukushima, N., et al. 1998. Peptides19, 811.
Gravitt, K.R. 1994. Biochem. Pharmacol. 47, 425.
Baudier, J., et al. 1987. Biochemistry26, 2886.
Kincaid, R.L., and Coulson, C.C. 1985. Biochem. Biophys. Res. Commun.133, 256.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

>97% (HPLC)

lyophilized

Calbiochem^®

OK to freeze

water: 1 mg/mL

ambient

−20℃

1S/C131H229N39O31/c1-23-71(16)102(163-97(176)60-135)122(194)146-62-98(177)148-74(19)109(181)164-100(69(12)13)124(196)160-88(55-65(4)5)116(188)155-84(41-30-33-51-134)115(187)165-101(70(14)15)125(197)161-90(57-67(8)9)118(190)168-106(77(22)173)128(200)169-105(76(21)172)123(195)147-63-99(178)150-92(58-68(10)11)129(201)170-54-36-44-94(170)121(193)149-75(20)108(180)158-89(56-66(6)7)117(189)166-104(73(18)25-3)127(199)162-93(64-171)120(192)159-91(59-78-61-145-80-38-27-26-37-79(78)80)119(191)167-103(72(17)24-2)126(198)157-83(40-29-32-50-133)111(183)154-85(42-34-52-143-130(139)140)112(184)152-82(39-28-31-49-132)110(182)153-86(43-35-53-144-131(141)142)113(185)156-87(46-48-96(137)175)114(186)151-81(107(138)179)45-47-95(136)174/h26-27,37-38,61,65-77,81-94,100-106,145,171-173H,23-25,28-36,39-60,62-64,132-135H2,1-22H3,(H2,136,174)(H2,137,175)(H2,138,179)(H,146,194)(H,147,195)(H,148,177)(H,149,193)(H,150,178)(H,151,186)(H,152,184)(H,153,182)(H,154,183)(H,155,188)(H,156,185)(H,157,198)(H,158,180)(H,159,192)(H,160,196)(H,161,197)(H,162,199)(H,163,176)(H,164,181)(H,165,187)(H,166,189)(H,167,191)(H,168,190)(H,169,200)(H4,139,140,143)(H4,141,142,144)

VDXZNPDIRNWWCW-UHFFFAOYSA-N

250 μg in Glass bottle
Yes

11 - Combustible Solids

WGK 3