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InSolution Y-27632-Calbiochem

产品编号:4145206
规格:Y-27632A, CAS 146986-50-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency.
包装规格:500 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4145206500 μG2210
产品别名

InSolution Y-27632-Calbiochem

Rho Kinase Inhibitor VI

基本信息
Empirical Formula【经验(实验)分子式】
C14H21N3O · 2HCl · xH2O
Molecular weight
320.26 (anhydrous basis)
General description【一般描述】
A highly potent, cell-permeable, selective inhibitor of Rho-associated protein kinase (Ki = 140 nM for p160ROCK (ROCK-I). Also inhibits ROCK-II with almost equal potency. Inhibition is achieved by competing with ATP for binding to the catalytic site. Exhibits 10- to 50-fold lower affinity for PKCε than p160ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at high concentrations (~100 mM). Also acts as a potent inhibitor of agonist-induced Ca2+ sensitization of myosin phosphorylation and smooth muscle contractions (IC50 = 700 nM for phenylephrine-induced contractions in aortic strips).
A 5 mM (500 µg/296 µl) aqueous solution of Y-27632 (Cat. No. 688000). A highly potent, cell-permeable, selective inhibitor of Rho-associated protein kinase (Ki = 140 nM for p160ROCK (ROCK-I). Also inhibits ROCK-II with almost equal potency. Inhibition is achieved by competing with ATP for binding to the catalytic site. Exhibits 10- to 50-fold lower affinity for PKCε than p160ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at high concentrations (~100 mM). Also acts as a potent inhibitor of agonist-induced Ca2+ sensitization of myosin phosphorylation and smooth muscle contractions (IC50 = 700 nM for phenylephrine-induced contractions in aortic strips).
Biochem/physiol Actions【生化/生理作用】
Target IC50: 700 nM against phenylephrine-induced contractions in aortic strips
Cell permeable: yes
Target Ki: 140 nM for p160ROCK (ROCK-I)
Reversible: no
Product competes with ATP.
Primary Target
p160ROCK
Warning【警告】
Toxicity: Harmful (C)
Physical form【外形】
A 5 mM (500 µg/296 µl) solution of Y-27632 (Cat. No. 688000) in H₂O.
Reconstitution【重悬】
Following initial thaw, aliquot and freeze (-20°C).
Other Notes【其他说明】
Chitaley, K., et al. 2001. Nat. Med.7, 119.
Davies, S.P., et al. 2000. Biochem. J. 351, 95.
Narumiya, S., et al. 2000. Methods Enzymol.325, 273.
Hirose, M., et al. 1999. J. Cell Biol.141, 1625.
Maekawa, M., et al. 1999. Science285, 895.
Uehata, M., et al. 1997. Nature389, 990.
Legal Information【法律信息】
Sold under license of PCT Application W098/06,433A1 and under license from Mitsubishi Pharma Tanabe Corporation.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
Assay【测定】
≥95% (HPLC)
form【形式】
liquid
manufacturer/tradename
Calbiochem®
storage condition【储存条件】
OK to freeze
protect from light
shipped in【运输】
wet ice
storage temp.【储存温度】
−20℃
packaging【包装】
500 μg in Plastic ampoule
Packaged under inert gas
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 2
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