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InSolution Y-27632-Calbiochem
产品编号: | 4145206 |
规格: | Y-27632A, CAS 146986-50-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency. |
包装规格: | 500 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
InSolution Y-27632-Calbiochem
Rho Kinase Inhibitor VI
基本信息
Empirical Formula【经验(实验)分子式】 | C14H21N3O · 2HCl · xH2O |
Molecular weight | 320.26 (anhydrous basis) |
General description【一般描述】 | A highly potent, cell-permeable, selective inhibitor of Rho-associated protein kinase (Ki = 140 nM for p160ROCK (ROCK-I). Also inhibits ROCK-II with almost equal potency. Inhibition is achieved by competing with ATP for binding to the catalytic site. Exhibits 10- to 50-fold lower affinity for PKCε than p160ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at high concentrations (~100 mM). Also acts as a potent inhibitor of agonist-induced Ca2+ sensitization of myosin phosphorylation and smooth muscle contractions (IC50 = 700 nM for phenylephrine-induced contractions in aortic strips). A 5 mM (500 µg/296 µl) aqueous solution of Y-27632 (Cat. No. 688000). A highly potent, cell-permeable, selective inhibitor of Rho-associated protein kinase (Ki = 140 nM for p160ROCK (ROCK-I). Also inhibits ROCK-II with almost equal potency. Inhibition is achieved by competing with ATP for binding to the catalytic site. Exhibits 10- to 50-fold lower affinity for PKCε than p160ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at high concentrations (~100 mM). Also acts as a potent inhibitor of agonist-induced Ca2+ sensitization of myosin phosphorylation and smooth muscle contractions (IC50 = 700 nM for phenylephrine-induced contractions in aortic strips). |
Biochem/physiol Actions【生化/生理作用】 | Target IC50: 700 nM against phenylephrine-induced contractions in aortic strips Cell permeable: yes Target Ki: 140 nM for p160ROCK (ROCK-I) Reversible: no Product competes with ATP. Primary Target p160ROCK |
Warning【警告】 | Toxicity: Harmful (C) |
Physical form【外形】 | A 5 mM (500 µg/296 µl) solution of Y-27632 (Cat. No. 688000) in H₂O. |
Reconstitution【重悬】 | Following initial thaw, aliquot and freeze (-20°C). |
Other Notes【其他说明】 | Chitaley, K., et al. 2001. Nat. Med.7, 119. Davies, S.P., et al. 2000. Biochem. J. 351, 95. Narumiya, S., et al. 2000. Methods Enzymol.325, 273. Hirose, M., et al. 1999. J. Cell Biol.141, 1625. Maekawa, M., et al. 1999. Science285, 895. Uehata, M., et al. 1997. Nature389, 990. |
Legal Information【法律信息】 | Sold under license of PCT Application W098/06,433A1 and under license from Mitsubishi Pharma Tanabe Corporation. CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
Assay【测定】 | ≥95% (HPLC) |
form【形式】 | liquid |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze protect from light |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −20℃ |
packaging【包装】 | 500 μg in Plastic ampoule Packaged under inert gas |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |