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FPTase, GST-Fusion, His·Tag®, Rat, Recombinant, E. coli

产品编号:4144722
规格:FPTase, GST-Fusion, His·Tag, Rat, Recombinant, E. coli, is composed of an74 kDa α- and 47 kDa β-subunit. The α-subunit is expressed as a GST-fusion construct with an N-terminal His·Tag sequence.
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4144722100 μG9560
产品别名

FPTase, GST-Fusion, His·Tag®, Rat, Recombinant, E. coli

FTase, Farnesyl Proteintransferase

产品性质
Quality Level【质量水平】
100
recombinant【重组】
expressed in E. coli
Assay【测定】
≥90% (SDS-PAGE)
form【形式】
liquid
manufacturer/tradename
Calbiochem®
storage condition【储存条件】
OK to freeze
avoid repeated freeze/thaw cycles
shipped in【运输】
wet ice
storage temp.【储存温度】
−70℃
packaging【包装】
100 μg in Plastic ampoule
基本信息
General description【一般描述】
Recombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence. Both the GST moiety and the His•Tag sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ and Mg2+ for optimal activity.
Recombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion protein with an N-terminal His•Tag sequence. Both the GST moiety and the His•Tag sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ and Mg2+ for optimal activity.
Biochem/physiol Actions【生化/生理作用】
1 pmol FTase will transfer ≥1 pmol farnesyl to H-Ras in 10 min at 37°C, pH 7.2.
Warning【警告】
Toxicity: Standard Handling (A)
Physical form【外形】
In 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
Reconstitution【重悬】
Following initial thaw, aliquot and freeze (-70°C).
Other Notes【其他说明】
Zimmerman, K.K., et al. 1998. Protein Expr. Purif.14, 395.
Reiss, Y., et al. 1990. Cell62, 81.
Legal Information【法律信息】
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 1
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