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Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli
| 产品编号： | 4144598 |
| 规格： | Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli cleaves asparagine-linked or free oligomannose and hybrid. Suitable for deglycosylation of native proteins. |
| 包装规格： | 700 MIU |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli
Endo-β-N-acetylglucosaminidase F1, Endo F1
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 3.2.1.96 ( BRENDA | IUBMB ) |
| General description【一般描述】 | Recombinant, Elizabethkingia meningosepticum endoglycosidase F1 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and hybrid, but not complex oligosaccharides. Core fucosylation reduces activity by 50 fold. Endo F1 will hydrolyze sulfate containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins. Recombinant, Elizabethkingia meningosepticum endoglycosidase F1 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and hybrid, but not complex oligosaccharides. Core fucosylation reduces activity by 50 fold. Endo F1 will hydrolyze sulfate containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185), and therefore is more suitable for deglycosylation of native proteins. Note: 1 mU = 1 milliunit. |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Unit Definition【单位定义】 | One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5. |
| Physical form【外形】 | In 20 mM Tris-HCl, pH 7.5. |
| Other Notes【其他说明】 | Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44. Tarentino, A.L., et al. 1992. J. Biol. Chem. 267, 3868. Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
| Quality Level【质量水平】 | 100 |
| recombinant【重组】 | expressed in E. coli |
| form【形式】 | liquid |
| Specific Activity | ≥16 units/mg protein ≥17 units/mL |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | do not freeze |
| foreign activity【异质活性】 | Proteases, none detected |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | 2-8℃ |
| packaging【包装】 | 700 miu in Glass bottle |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
