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泛素小鼠单克隆抗体(FK2)

Anti-Ubiquitin Mouse mAb (FK2)
产品编号:4144070
规格:liquid, clone FK2, Calbiochem®
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4144070100 μG5850
产品别名

泛素小鼠单克隆抗体(FK2)

Anti-Ubiquitin Mouse mAb (FK2)

产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
unpurified
antibody product type
primary antibodies
clone【克隆】
FK2, monoclonal
form【形式】
liquid
contains【包含】
≤0.1% sodium azide as preservative
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition【储存条件】
OK to freeze
avoid repeated freeze/thaw cycles
isotype【同位素/亚型】
IgG1
shipped in【运输】
wet ice
storage temp.【储存温度】
−20℃
packaging【包装】
100 μg in Glass bottle
Please refer to vial label for lot-specific concentration.
基本信息
General description【一般描述】
This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin.
Recognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins, but not free ubiquitin, in Raji cells.
Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins.
Immunogen【免疫原】
unpurified polyubiquitinylated lysozyme
human
Application【应用】

ELISA (see application references)
Immunoblotting (1:1000)
Immunoprecipitation (see application references and comments)
Immunofluorescence (see application references)
Warning【警告】
Toxicity: Standard Handling (A)
Physical form【外形】
In PBS.
Preparation Note【制备说明】
Raji cells
Reconstitution【重悬】
Following initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.
Other Notes【其他说明】
Matsumoto, M., et al. 2005. Proteomics 5, 4545.
Boname, J.N., et al. 2001. Immunity 15, 627.
Campbell, V., et al. 2001. Neuron 32, 1013.
Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
Does not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.
Legal Information【法律信息】
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 2
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