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泛素小鼠单克隆抗体(FK2)
Anti-Ubiquitin Mouse mAb (FK2)
产品别名
泛素小鼠单克隆抗体(FK2)
Anti-Ubiquitin Mouse mAb (FK2)
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | unpurified |
antibody product type | primary antibodies |
clone【克隆】 | FK2, monoclonal |
form【形式】 | liquid |
contains【包含】 | ≤0.1% sodium azide as preservative |
species reactivity (predicted by homology) | all |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
isotype【同位素/亚型】 | IgG1 |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −20℃ |
packaging【包装】 | 100 μg in Glass bottle Please refer to vial label for lot-specific concentration. |
基本信息
General description【一般描述】 | This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin. Recognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins, but not free ubiquitin, in Raji cells. Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins. |
Immunogen【免疫原】 | unpurified polyubiquitinylated lysozyme human |
Application【应用】 | ELISA (see application references) Immunoblotting (1:1000) Immunoprecipitation (see application references and comments) Immunofluorescence (see application references) |
Warning【警告】 | Toxicity: Standard Handling (A) |
Physical form【外形】 | In PBS. |
Preparation Note【制备说明】 | Raji cells |
Reconstitution【重悬】 | Following initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month. |
Other Notes【其他说明】 | Matsumoto, M., et al. 2005. Proteomics 5, 4545. Boname, J.N., et al. 2001. Immunity 15, 627. Campbell, V., et al. 2001. Neuron 32, 1013. Fujimuro, M., et al. 2003. FEBS Letts. 349, 173. Does not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |