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Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1)

产品编号:4144050
规格:liquid, clone 7-6C1, Calbiochem®
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4144050100 μG5850
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
7-6C1, monoclonal
form【形式】
liquid
contains【包含】
≤0.1% sodium azide as preservative
species reactivity
human, rat, bovine
should not react with
mouse
manufacturer/tradename
Calbiochem®
storage condition【储存条件】
OK to freeze
avoid repeated freeze/thaw cycles
isotype【同位素/亚型】
IgG1
shipped in【运输】
wet ice
storage temp.【储存温度】
−20℃
packaging【包装】
100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
基本信息
General description【一般描述】
Purified mouse monoclonal antibody (see application references). Recognizes the ~28 kDa TIMP-1 protein.
Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an approximately 10 kDa segment from the N-terminus and their activity is regulated by endogenous inhibitors. These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis.The activation and activity of MMPs are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP-1 (also called EPA, Fibroblast collagenase inhibitor or collagenase inhibitor) is a 28 kDa glycoprotein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-1 preferentially binds and inhibits MMP-9. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models.
This Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TIMP-1 (Ab-1).
Recognizes the ~28.5 kDa TIMP-1 protein. Also recognizes MMP/TIMP-1 complexes.
Immunogen【免疫原】
Bovine
purified, bovine dental pulp TIMP-1
Application【应用】

Immunoblotting (1 g/ml, see application references)
Immunocytochemistry (see application references)
Paraffin Sections (25 g/ml, see application references, see comments)
Warning【警告】
Toxicity: Standard Handling (A)
Physical form【外形】
In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
Reconstitution【重悬】
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note【分析说明】
Positive Control
rTIMP-1 Protein (Cat. Nos. PF019 or PF020)
Other Notes【其他说明】
Fujimoto, N. and Iwata, K., 1994. Connective Tissue26, 237.
Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol.2, 861.
Stetler-Stevenson, W. G., et al. 1993. FASEB J.7, 1434.
Woessner, J. F., 1991. FASEB J.5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology ed. M. M. Gottesman. Vol. 99-106.
Also recognizes TIMP-1/MMP complexes.This antibody has been used to stain paraffin sections fixed with PLP (perioddate-lysine-4% paraformaldehyde) and 10% formaldehyde (using 25 µg/ml antibody). Antibody should be titrated for optimal results in individual systems.
Legal Information【法律信息】
Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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