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Anti-Sox2 Mouse mAb (245610)
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | 245610, monoclonal |
form【形式】 | lyophilized |
species reactivity | mouse, human |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze |
isotype【同位素/亚型】 | IgG2a |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −20℃ |
packaging【包装】 | 100 μg in Plastic ampoule |
基本信息
General description【一般描述】 | Protein G purified mouse monoclonal antibody derived by immunizing mice with the specified immunogen and fusing splenocytes with mouse myeloma cells. Recognizes the ~34 kDa Sox2 protein. This Anti-Sox2 Mouse mAb (245610) is validated for use in Immunoblotting, Immunocytochemistry, Flow Cytometry for the detection of Sox2. Recognizes the ~34 kDa Sox2 protein in NTERA-2 cells. |
Immunogen【免疫原】 | a recombinant protein consisting of amino acids 135-317 of human Sox2, expressed in E. coli Human |
Application【应用】 | Immunoblotting (1-2 µg/ml) Immunocytochemistry (10 µg/ml; see comments) Flow Cytometry (see comments) |
Warning【警告】 | Toxicity: Standard Handling (A) |
Physical form【外形】 | Lyophilized from 0.2 µm-filter sterilized PBS, 5% trehalose. |
Reconstitution【重悬】 | Reconstitute with 200 µl sterile PBS for a final stock concentration of 500 µg/ml. Following reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage. Avoid freeze/thaw cycles of solutions. Thawed aliquots are stable for up to 1 month at 4°C. |
Analysis Note【分析说明】 | Positive Control NTERA-2 cells |
Other Notes【其他说明】 | Avilion, A.A., et al. 2003.Genes Dev.17, 126. Graham, V., et al. 2003.Neuron39, 749. Stevanovic, M. 2003.Mol. Biol. Rep.30, 127. Kishi, M., et al. 2000.Development127, 791. Uwanogho, D., et al. 1995.Mech. Dev.49, 23. Yuan, H., et al. 1995.Genes Dev.9, 2635. For immunocytochemistry, cells should be fixed in PBS containing 4% paraformaldehyde for 20 min, followed by blocking in PBS/10% normal donkey serum/1% BSA/0.1% Triton™ X-100 detergent for 45 min and staining overnight at 4°C with appropriately diluted primary antibody. For immunoblotting, the best results may be obtained using whole cell or nuclear extracts; Sox2 may be difficult to detect in cytoplasmic extracts. The detection limit for recombinant Sox2 is ~5 ng/lane under reducing and non-reducing conditions. For intracellular staining by flow cytometry, fix cells in 4% paraformaldehyde and permeabilize with 0.1% saponin. Following fixation and permeabilization, dilute the antibody to 50 µg/ml and add 10 µl diluted antibody to 1-2.5 x 105 cells in a total volume of ≤200 µl. Following incubation with appropriate detection antibody, the cells should be washed a final time with 0.1% saponin prior to analysis. Antibody should be titrated for optimal results in individual systems. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 1 |