您的位置：首页 > 产品中心 > Anti-N-Myc Mouse mAb (NCM II 100)
Anti-N-Myc Mouse mAb (NCM II 100)
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | NCM II 100, monoclonal |
| form【形式】 | liquid |
| does not contain【不包含】 | preservative |
| species reactivity | human, mouse |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
| isotype【同位素/亚型】 | IgG1 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
| General description【一般描述】 | This Anti-N-Myc Mouse mAb (NCM II 100) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of N-Myc. Recognizes the N-myc protein and its cleavage product in IMR5 cells. Purified mouse monoclonal antibody generated by immunizing BALB/c mice with specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells (see application references). Recognizes N-myc and its cleavage products. |
| Immunogen【免疫原】 | Human recombinant, human, N-myc fusion protein, expressed in E. coli |
| Application【应用】 | Frozen Sections (1-5 µg/ml) Immunoblotting (1-5 µg/ml, see application references) Immunofluorescence (1-5 µg/ml, see application references) Immunoprecipitation (1-2 µg/sample, see application references) |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Physical form【外形】 | In 50 mM sodium phosphate buffer, 50% glycerol. |
| Reconstitution【重悬】 | Following initial thaw, aliquot and freeze (-20°C). |
| Analysis Note【分析说明】 | Positive Control IMR5 cells |
| Other Notes【其他说明】 | It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. Antibody should be titrated for optimal results in individual systems. Brondyk, W.H. 1991. Oncogene6, 1269. Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research.2, 133. LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144. Cole, M.D., 1986. Ann. Rev. Gen.20, 361. Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA83, 5929. Slamon, D.J., et al. 1986. Science232, 768. Seeger, R.C., et al. 1985. New Engl. J. Med.313, 1111. Brodeur, G.M., et al. 1984. Science224, 1121. Michitsch, R.W., et al. 1984. Mol. Cell. Biol.11, 2370. Kohl, N.E., et al. 1983. Cell35, 603. Schwab, M., et al. 1983. Nature305, 245. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
