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Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2)
产品别名
Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2)
Anti-His6
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | 13/45/31/2, monoclonal |
form【形式】 | liquid |
contains【包含】 | ≤0.1% sodium azide as preservative |
species reactivity (predicted by homology) | all |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | do not freeze |
isotype【同位素/亚型】 | IgG1 |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | 2-8℃ |
packaging【包装】 | 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
General description【一般描述】 | Anti-Histidine-Tagged Protein, mouse monoclonal, clone 13/45/31/2, specifically recognizes an epitage of 6 consecutive His residues. It is validated for use in ELISA, WB, IF & IP. Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes a His•Tag Specifically recognizes an epitage of six consecutive His residues of both natural and recombinant sources. |
Immunogen【免疫原】 | Epitope: HHHHHH a recombinant protein containing a His•Tag |
Application【应用】 | ELISA (0.1-0.4 g/ml) Immunoblotting (1-4 g/ml) Immunofluorescence (1-4 g/ml) Immunoprecipitation (4 g/ml) |
Warning【警告】 | Toxicity: Standard Handling (A) |
Physical form【外形】 | In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5. |
Analysis Note【分析说明】 | Positive Control Proteins containing a His•Tag |
Other Notes【其他说明】 | Zentgraf, H., et al. 1995. Nucl. Acids. Res.23, 3347. Gu, J., et al. 1994. BioTechniques17, 257. Sporeno, E., et al. 1994. J. Biol. Chem., 10991. Sisk, W.P., et al. 1994. J. Virol.68, 766. Lu, T., et al. 1993. Anal. Biochem.213, 318. Garner, J., et al. 1992. Cell69, 833. Hochuli, E., et al. 1987. J. Chromatogr.411, 177. Antibody should be titrated for optimal results in individual systems. |
Legal Information【法律信息】 | Manufactured by Dianova, GmbH, Germany. CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 1 |