Anti-BRCA2 (Ab-1) Mouse mAb (2B)

100

mouse

purified antibody

primary antibodies

2B, monoclonal

liquid

≤0.1% sodium azide as preservative

human

Calbiochem^®

OK to freeze

IgG2b

wet ice

−20℃

100 μg in Glass bottle
Please refer to vial label for lot-specific concentration.

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes the ~460 kDa BRCA2 protein.
Anti-BRCA2 (Ab-1), mouse monoclonal, clone 2B, recognzies the ~460 kDa BRCA2 protein in cells expressing a wild type BRCA2 gene. It is validated for Western blotting and immunoprecipitation.
Recognzies the ~460 kDa BRCA2 protein in cells expressing a wild type BRCA2 gene. Sold under license of U.S. Patent 5,753,441 and 6,162,897.

a recombinant protein consisting of amino acids 1651-1821 of human BRCA2 fused to GST
Human

Immunoblotting (2 µg/ml)

Immunoprecipitation (2 µg)

Toxicity: Standard Handling (A)

In PBS.

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
MCF7 cells

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The CAPAN-1 cell line, which has 6174delT mutation in the single BRCA2 allele, expresses a truncated BRCA2 protein of 230 kDa that contains the Anti-BRCA2 epitope and retains RAD51 binding but has lost p53 binding. Full length BRCA2 protein will co-migrate on SDS-PAGE with the catalytic subunit of DNA PK which can be detected using Anti-DNA PK, Cat. No. PC127. Antibody should be titrated for optimal results in individual systems.

Sold under license of U.S. Patents 5,837,492 and 6,124,104.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

11 - Combustible Solids

WGK 1