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Anti-AnkyrinG Mouse mAb (463)
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | 463, monoclonal |
| Assay【测定】 | ≥95% (SDS-PAGE) |
| form【形式】 | liquid |
| contains【包含】 | ≤0.1% sodium azide as preservative |
| species reactivity | mouse, canine, rat, human |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | do not freeze |
| isotype【同位素/亚型】 | IgG1 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | 2-8℃ |
| packaging【包装】 | 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
| General description【一般描述】 | Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63/Ag 8.653 mouse myeloma cells. Recognizes the ~190 kDa isoform of AnkyrinG. Anti-Ankyrin G, mouse monoclonal, clone 463, recognizes the ~190 kDa ankyrin G protein in brain extracts. It is validated for Western blotting, immunofluorescence, and frozen sections. Recognizes the ~190 kDa ankyrinG protein in brain extracts. Reacts with all spliced forms of ankyrinG. |
| Immunogen【免疫原】 | a synthetic peptide corresponding to amino acids from the spectrin-binding domain of human ankyrinG Human |
| Application【应用】 |  Frozen Sections (1-2 g/ml) Immunoblotting (2-5 g/ml) Immunofluorescence (1-2 g/ml) |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Physical form【外形】 | In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5. |
| Analysis Note【分析说明】 | Positive Control Mouse or rat brain extract or unmyelinated axons |
| Other Notes【其他说明】 | AnkyrinG is a good immunohistochemical marker for axon initial segments, nodes of Ranvier, and epithelial cell-cell contact sites. Antibody should be titrated for optimal results in individual systems. Davis, J.Q., et al. 1995. Mol. Biol. Cell6, 177a, supplement. Kunimoto, M., et al. 1995. FEBS Lett.357, 217. Lambert, S., et al. 1995. Mol. Biol. Cell6, 98a supplement. Bennett, V. and Gilligan, D.M. 1993. Ann. Rev. Cell Biol.9, 27. Kunimoto, M., et al. 1991. J. Cell Biol.115, 1319. Otto, E., et al. 1991. J. Cell Biol.114, 241. Davis, J., and Bennett, V. 1984. J. Biol. Chem.259, 13550. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | nwg |
