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Anti-v-Src (Ab-1) Mouse mAb (327)

产品编号:4142159
规格:liquid, clone 327, Calbiochem®
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4142159100 μG5520
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
327, monoclonal
form【形式】
liquid
contains【包含】
≤0.1% sodium azide as preservative
species reactivity
human, mouse, rat
manufacturer/tradename
Calbiochem®
storage condition【储存条件】
do not freeze
isotype【同位素/亚型】
IgG1
shipped in【运输】
wet ice
storage temp.【储存温度】
2-8℃
packaging【包装】
100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
基本信息
General description【一般描述】
Recognizes the ~60 kDa v-Src and c-Src proteins in NIH/3T3 and HT1080 cells. May appear as a doublet due to phosphorylation.
Purified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 myeloma cells (see application references). Recognizes the ~60 kDa Src protein.
This Anti-v-Src (Ab-1) Mouse mAb (327) is validated for use in Immunoblotting, Immunoprecipitation, Frozen Sections, Immunofluorescence, Paraffin Sections for the detection of v-Src (Ab-1).
Immunogen【免疫原】
purified v-Src
Epitope: within the SH3 domain of v-Src and c-Src
Application【应用】
Immunoblotting (2.5 µg/ml, see application references)

Immunoprecipitation (1 µg antibody or use Cat. No. OP07A; see application references)

Frozen Sections (not recommended)

Immunofluorescence (not recommended)

Paraffin Sections (not recommended)
Warning【警告】
Toxicity: Standard Handling (A)
Physical form【外形】
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.
Analysis Note【分析说明】
Positive Control
NIH/3T3 cells
Other Notes【其他说明】
pp60src may appear as a doublet due to phosphorylation. This antibody can be used to precipitate active src kinase (see application references). The agarose conjugate, Cat. No. OP07A, is available separately for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Courtneidge, S.A. and Heber, A. 1987. Cell50, 1031.
Cartwright, C.A., et al. 1985. Mol. Cell. Biol.5,, 2647.
Courtneidge, S.A. 1985. EMBO J.4, 1471.
Whitman, M., et al. 1985. Nature315, 239
Bolen, J.B., et al. 1984. Cell38, 767.
Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci.77, 1311.
Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci.76, 3159.
Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci.75, 2021.
Collett, M.S., et al. 1978. Cell15, 1363.
Legal Information【法律信息】
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
nwg
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