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Anti-CD18 Mouse mAb (IB4)
产品别名
Anti-CD18 Mouse mAb (IB4)
Anti-β2 Integrin
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | IB4, monoclonal |
Assay【测定】 | ≥95% (SDS-PAGE) |
form【形式】 | liquid |
contains【包含】 | 0.5 mg/mL gentamicin sulfate as preservative |
species reactivity | human |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze |
isotype【同位素/亚型】 | IgG2a |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | 2-8℃ |
packaging【包装】 | 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
General description【一般描述】 | Anti-CD18, mouse monoclonal, clone IB4, recognizes the ~95 kDa β2 integrin subunit. It is validated for FC, IP, IHC on frozen sections, and cell inhibition assays. Protein A purified mouse monoclonal antibody. Recognizes the ~95 kDa β2 integrin subunit (CD18). Recognizes the ~95 kDa β2 integrin subunit. Blocks the binding of ICAM-1 and ICAM-3 to LFA-1. |
Immunogen【免疫原】 | Human human peripheral blood monocytes |
Application【应用】 | Flow Cytometry (5-10 µg/ml) Frozen Sections (see comments) Immunoprecipitation (see comments) Cell Inhibition Assays (see comments) |
Warning【警告】 | Toxicity: Standard Handling (A) |
Physical form【外形】 | In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5. |
Reconstitution【重悬】 | Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles. |
Analysis Note【分析说明】 | Negative Control Nalm-6 cells Positive Control HPB-MLT cells |
Other Notes【其他说明】 | Berman, M.E., et al. 1996. J. Immunol.156, 1515. Kaneko, M., et al. 1995. J. Immunol. 155, 2631. Mazzone, A., and Ricevuti, G. 1995. Haematologica80, 161. Kaneko, M., et al. 1995. J. Immunol.155, 2631. Landis, R.C., et al. 1994. J. Cell Biol.126, 529. Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5699. Blocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |