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Anti-CD18 Mouse mAb (IB4)

产品编号:4142150
规格:liquid, ≥95% (SDS-PAGE), clone IB4
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4142150100 μG5210
产品别名

Anti-CD18 Mouse mAb (IB4)

Anti-β2 Integrin

产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
IB4, monoclonal
Assay【测定】
≥95% (SDS-PAGE)
form【形式】
liquid
contains【包含】
0.5 mg/mL gentamicin sulfate as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition【储存条件】
OK to freeze
isotype【同位素/亚型】
IgG2a
shipped in【运输】
wet ice
storage temp.【储存温度】
2-8℃
packaging【包装】
100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
基本信息
General description【一般描述】
Anti-CD18, mouse monoclonal, clone IB4, recognizes the ~95 kDa β2 integrin subunit. It is validated for FC, IP, IHC on frozen sections, and cell inhibition assays.
Protein A purified mouse monoclonal antibody. Recognizes the ~95 kDa β2 integrin subunit (CD18).
Recognizes the ~95 kDa β2 integrin subunit. Blocks the binding of ICAM-1 and ICAM-3 to LFA-1.
Immunogen【免疫原】
Human
human peripheral blood monocytes
Application【应用】
Flow Cytometry (5-10 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

Cell Inhibition Assays (see comments)
Warning【警告】
Toxicity: Standard Handling (A)
Physical form【外形】
In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Reconstitution【重悬】
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Analysis Note【分析说明】
Negative Control
Nalm-6 cells
Positive Control
HPB-MLT cells
Other Notes【其他说明】
Berman, M.E., et al. 1996. J. Immunol.156, 1515.
Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
Mazzone, A., and Ricevuti, G. 1995. Haematologica80, 161.
Kaneko, M., et al. 1995. J. Immunol.155, 2631.
Landis, R.C., et al. 1994. J. Cell Biol.126, 529.
Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5699.
Blocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information【法律信息】
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 1
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