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Anti-c-Abl (Ab-3) Mouse mAb (24-21)
产品别名
Anti-c-Abl (Ab-3) Mouse mAb (24-21)
Anti-p150, Anti-Abl
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | 24-21, monoclonal |
form【形式】 | liquid |
contains【包含】 | ≤0.1% sodium azide as preservative |
species reactivity | human, mouse |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | do not freeze |
isotype【同位素/亚型】 | IgG1 |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | 2-8℃ |
packaging【包装】 | 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
General description【一般描述】 | Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with p.3U1 mouse myeloma cells (see application references). Recognizes the v-Abl, the ~145-150 kDa c-Abl, the ~210 kDa Bcr-Abl (CML), and the ~190 kDa Bcr-Abl (ALL) proteins. Anti-c-Abl (Ab-3), mouse monoclonal, clone 24-21, recognizes the ~145 kDa human and ~150 kDa mouse c-Abl. It is validated for Western blotting, immunofluorescence, and immunoprecipitation. Recognizes the ~145 kDa human c-Abl, the ~150 kDa mouse c-Abl, v-Abl, and the Bcr/Abl translocation products in ALL (~190 kDa) and CML (~210 kDa). Does not inhibit protein tyrosine kinase activity. |
Immunogen【免疫原】 | a recombinant protein consisting of the carboxyl region of v-abl protein fused to TrpE |
Application【应用】 | Immunoblotting (1-5 µg/ml) Immunofluorescence (see application references) Immunoprecipitation (1-2 µg/sample) Neutralization Studies (not recommended) |
Warning【警告】 | Toxicity: Standard Handling (A) |
Physical form【外形】 | In 0.05 M sodium phosphate buffer, 0.2% gelatin, pH 7.5. |
Analysis Note【分析说明】 | Positive Control K562 (human bcr/abl), HL-60 (normal abl), and ANN-1 (murine abl) cells |
Other Notes【其他说明】 | Does not inhibit protein tyrosine kinase activity. Detects human c-Abl (145 kDa), mouse c-Abl (150 kDa), v-Abl, and the Bcr/Abl translocation products in ALL (~210 kDa) and CML (~190 kDa). HL-60 and NIH3T3 cells contain normal, non-rearranged protein. Antibody should be titrated for optimal results in individual sample types. Wiedemann, L.M., et al. 1988. Blood71, 349. Stam, K., et al. 1985. N. Engl. J. Med.313, 1429. Konopka, J.B., et al. 1984. Cell37, 1035. Prywes, R., et al. 1983. Cell34, 569. de Klein, A., et al. 1982. Nature300, 765. Reynolds, F.H., Jr., et al. 1980. J. Virol.36, 374. Reynolds, F.H., Jr., et al. 1978. Proc. Natl. Acad. Sci. USA75, 3974. Witte, O.N., et al. 1978. Proc. Natl. Acad. Sci. USA75, 2488. Abelson, H.T. and Rabstein, L.S. 1970. Cancer Res.30, 2213. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | nwg |