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Dulbecco′s Modified Eagle′s Medium-high glucose
产品编号: | 4142096 |
规格: | With 4500 mg/L glucose, L-alanyl-glutamine, and sodium bicarbonate, without sodium pyruvate., liquid, sterile-filtered, suitable for cell culture |
包装规格: | 500 ML |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品性质
Quality Level【质量水平】 | 400 |
sterility【无菌性】 | sterile-filtered sterile |
form【形式】 | liquid |
technique(s) | cell culture | mammalian: suitable |
shipped in【运输】 | ambient |
storage temp.【储存温度】 | 2-8℃ |
基本信息
Components【组分】 | stable glutamine: yes phenol red: yes HEPES: no NaHCO3: yes glucose: high sodium pyruvate: no |
General description【一般描述】 | This DMEM-Hi glucose medium differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. |
Application【应用】 | Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High". |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |