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XIAP Active human
产品编号: | 4141567 |
规格: | recombinant, expressed in baculovirus infected insect cells, ≥65% (SDS-PAGE) |
包装规格: | 20 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
XIAP Active human
X-linked inhibitor of apoptosis
Inhibitor of apoptosis protein 3
Baculoviral IAP repeat-containing protein 4 (BIRC4)
E3 ubiquitin-protein ligase XIAP (MIHA)
IAP-like protein (ILP1)
基本信息
NACRES | NA.32 |
General description【一般描述】 | XIAP (X-linked inhibitor of apoptosis) is an anti-apoptotic protein belonging to the inhibitor of apoptosis protein (IAP) family, an evolutionary conserved protein family. XIAP contains three tandem copies of BIR (baculovirusIAP repeat) domain, which is composed of ∼70 residues. IAP family members also contain a RING (really interesting new gene) zinc finger domain in their C-terminals. |
Application【应用】 | Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Biochem/physiol Actions【生化/生理作用】 | XIAP (X-linked inhibitor of apoptosis) is the most potent caspase-interacting protein and inhibits both intrinsic and extrinsic pathways of apoptosis. Its up-regulation in tumor cells is linked with resistance to chemotherapy. In inflammatory breast cancer (IBC) this protein is linked with resistance to antibody-dependent cellular cytotoxicity (ADCC) driven by cetuximab and trastuzumab. Embelin, an inhibitor of XIAP, functions as an anti-tumor agent in myeloma cell line (HL-60) by suppressing the expression of XIAP. Elevated expression of XIAP in rectal cancer is associated with chemotherapy resistance. |
Unit Definition【单位定义】 | One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37℃. |
Physical form【外形】 | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol, and 3 mM DTT. |
Preparation Note【制备说明】 | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles. |
产品性质
biological source【生物来源】 | human |
recombinant【重组】 | expressed in baculovirus infected insect cells |
Assay【测定】 | ≥65% (SDS-PAGE) |
form【形式】 | aqueous solution |
mol wt【分子量】 | 57 kDa |
packaging【包装】 | pkg of 20 μg |
storage condition【储存条件】 | avoid repeated freeze/thaw cycles |
NCBI accession no.【NCBI登记号】 | NM_001167 |
UniProt accession no.【UniProt登记号】 | P98170 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −70℃ |
Gene Information | human ... XIAP(331) |