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TRAIL, Soluble human
| 产品编号： | 4141090 |
| 规格： | recombinant, expressed in E. coli, His tagged, >98% (SDS-PAGE) |
| 包装规格： | 20 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
TRAIL, Soluble human
TRAIL
CD253
CD339
Apo-2L
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in E. coli |
| tag【标签】 | His tagged |
| Assay【测定】 | >98% (SDS-PAGE) |
| form【形式】 | liquid |
| mol wt【分子量】 | ~26 kDa |
| packaging【包装】 | pkg of 20 μg |
| concentration【浓度】 | ≥0.2 mg/mL |
| technique(s) | activity assay: suitable cell culture | mammalian: suitable transfection: suitable |
| impurities【杂质】 | <0.01 EU/μg endotoxin, tested |
| color【颜色】 | clear |
| UniProt accession no.【UniProt登记号】 | P50591 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... TNFSF10(8743) |
基本信息
| General description【一般描述】 | TNF-related apoptosis-inducing ligand (TRAIL; Apo2L; CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines, TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. The gene encoding TRAIL is localized on human chromosome 3q26.31. |
| Application【应用】 | TRAIL, soluble human has been used in cell culture, chemical transfection and in caspase-3/7 activity assay. |
| Biochem/physiol Actions【生化/生理作用】 | Trimerized TNF-related apoptosis-inducing ligand (TRAIL) triggers apoptosis upon ligation of cell surface TRAIL-receptor 1 (R1) and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC). Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-receptors 1,-2,-3,-4 {TRAIL-R1 (DR4; Apo2; CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A; TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1; LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D)} and osteoprotegerin (OPG; OCIF; TNFRSF11B). |
| Physical form【外形】 | Solution in 20 mM HEPES, pH 7.9, 120 mM NaCl, 0.01% Tween 20, 1% sucrose, 1 mM DTT. |
| Other Notes【其他说明】 | The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
