TIGAR human

NA.32

TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2-6 bisphosphate (F-2-6-BP) to fructose-6-phosphate and inorganic phosphate. F-2-6-BP is a powerful activator of 6-phosphofructose-1 kinase, the rate limiting enzyme of glycolysis. By lowering the intracellular level of F-2-6-BP, TIGAR expression leads to increased glucose processing via the pentose phosphate pathway, the major cellular source for NADPH. Recombinant human TIGAR expressed in E. coli is a 29.9 kDa protein containing 269 amino-acid residues.

Sterile filtered through a 0.2 micron filter. Lyophilized from 20 mM Tris, pH 8.0, 200 mM NaCl and 1 mM DTT.

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20℃ to -80℃.

human

expressed in E. coli

≥95% (SDS-PAGE)

lyophilized powder

29.9 kDa

pkg of 10 μg
pkg of 50 μg

<1 EU/μg endotoxin (LAL test)

Q9NQ88

wet ice

−20℃

human ... TIGAR(57103)

GHS07

Warning

H319

P264 - P280 - P305 + P351 + P338 - P337 + P313

Eye Irrit. 2

11 - Combustible Solids

WGK 3