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T4 Beta-glucosyltransferase

产品编号:4140841
规格:recombinant, expressed in E. coli, ≥83% (SDS-PAGE)
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4140841100 μG6990
产品别名

T4 Beta-glucosyltransferase

hydroxymethyl cytosine

UDP glucose-DNA ?-glucosyltransferase

T4-phage beta-glucosyltransferase

基本信息
NACRES
NA.32
General description【一般描述】
T4-phage β−glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in Escherichia coli.
Application【应用】
Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.
Biochem/physiol Actions【生化/生理作用】
T4-phage β-glucosyltransferase is involved in the transfer of glucose from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA of the T4-phage. Ions such as Mg2+, Mn2+ and Ca2+ activate this enzyme.
Physical form【外形】
Formulated in 200 mM imidazole and 20% glycerol.
产品性质
biological source【生物来源】
Escherichia coli
recombinant【重组】
expressed in E. coli
Assay【测定】
≥83% (SDS-PAGE)
form【形式】
aqueous solution
mol wt【分子量】
41.6 kDa
packaging【包装】
pkg of 100 μg
NCBI accession no.【NCBI登记号】
NP_049658
UniProt accession no.【UniProt登记号】
P04547
shipped in【运输】
dry ice
storage temp.【储存温度】
−70℃
Gene Information
bacteriophage T4 ... B-GT(1258765)
安全信息
Pictograms【象形图】
GHS07,GHS08
Signal word【警示用语:】
Danger
Hazard Statements
H315 - H319 - H360D
Precautionary Statements
P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
Hazard Classifications【危险分类】
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Storage Class Code【储存分类代码】
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 1
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