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SUMO Protease, Biotin tagged
产品编号: | 4140810 |
规格: | Recombinant protein, aqueous solution, ≥25,000 units/mL |
包装规格: | 2500 UNITS |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
SUMO Protease, Biotin tagged
Small Ubiquitin-like Modifier Protease
ULP
Ubiquitin-homology domain protein PIC1
Ubiquitin like protease
Ubl-specific protease 1
产品性质
recombinant【重组】 | expressed in E. coli |
Assay【测定】 | ≥90% (SDS-PAGE) |
form【形式】 | aqueous solution |
mol wt【分子量】 | 27 kDa |
concentration【浓度】 | ≥25,000 units/mL |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
基本信息
General description【一般描述】 | SUMO proteases are enzymes that specifically cleave the post-translational protein modification (PTM) known as small ubiquitin-related modifier (SUMO). SUMO falls into the PTM class of ubiquitin and/or ubiquitin-like proteins (UBL).SUMO protease is the Ubl-specific protease 1 (Ulp1) from Saccharomyces cerevisiae. This was the first of this class of enzymes to be isolated. SUMO protease cleaves specifically the SUMO moiety in a ‘scarless′ manner. After recognizing the tertiary structure of the Ubiquitin-like SUMO domain, SUMO protease hydrolyzes the peptide bond in the x–Gly–Gly–x sequence after the Gly-Gly bond, at the C-terminus of the SUMO domain. Besides the cleavage of natural SUMO-modified proteins, SUMO protease is used to cleave recombinant SUMO fusion proteins. The SUMO domain is a known solubility-enhancing fusion tag used in recombinant protein expression. Since this recombinant protease does not contain any coman protein purification tag it can be used for on-column cleavage of column bound SUMO fusion protein. Sumo protease with Biotin tag can be easily removed at the end of the digestion reaction. |
Application【应用】 | This biotin-tagged SUMO protease product is designed to be used for on-column cleavage of SUMO fusion proteins. This method specifically cleaves the protein of interest from a column-bound SUMO fusion protein, leaving the SUMO domain bound to the affinity column (e.g. Ni-NTA column) and eluting only the protein of interest. This method is advantageous to post-elution cleavage for several reasons: Eliminates most of the impurities normally associated with purification on Ni-chelating columns.
This biotin-tagged SUMO protease has been enzymatically biotinylated without affecting its proteolytic activity. It does not include any additional protein purification tag (e.g., histidine-tag or GST). |
Unit Definition【单位定义】 | One enzyme unit is defined as the amount that will cut 90% of 100 pmol of SUMO-GST in 1 hour at 30℃. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 2 |