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SUMO Protease, Biotin tagged

产品编号:4140810
规格:Recombinant protein, aqueous solution, ≥25,000 units/mL
包装规格: 2500 UNITS
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4140810 2500 UNITS5070
产品别名

SUMO Protease, Biotin tagged

Small Ubiquitin-like Modifier Protease

ULP

Ubiquitin-homology domain protein PIC1

Ubiquitin like protease

Ubl-specific protease 1

产品性质
recombinant【重组】
expressed in E. coli
Assay【测定】
≥90% (SDS-PAGE)
form【形式】
aqueous solution
mol wt【分子量】
27 kDa
concentration【浓度】
≥25,000 units/mL
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
基本信息
General description【一般描述】
SUMO proteases are enzymes that specifically cleave the post-translational protein modification (PTM) known as small ubiquitin-related modifier (SUMO). SUMO falls into the PTM class of ubiquitin and/or ubiquitin-like proteins (UBL).SUMO protease is the Ubl-specific protease 1 (Ulp1) from Saccharomyces cerevisiae. This was the first of this class of enzymes to be isolated. SUMO protease cleaves specifically the SUMO moiety in a ‘scarless′ manner. After recognizing the tertiary structure of the Ubiquitin-like SUMO domain, SUMO protease hydrolyzes the peptide bond in the x–Gly–Gly–x sequence after the Gly-Gly bond, at the C-terminus of the SUMO domain. Besides the cleavage of natural SUMO-modified proteins, SUMO protease is used to cleave recombinant SUMO fusion proteins. The SUMO domain is a known solubility-enhancing fusion tag used in recombinant protein expression. Since this recombinant protease does not contain any coman protein purification tag it can be used for on-column cleavage of column bound SUMO fusion protein. Sumo protease with Biotin tag can be easily removed at the end of the digestion reaction.
Application【应用】
This biotin-tagged SUMO protease product is designed to be used for on-column cleavage of SUMO fusion proteins. This method specifically cleaves the protein of interest from a column-bound SUMO fusion protein, leaving the SUMO domain bound to the affinity column (e.g. Ni-NTA column) and eluting only the protein of interest. This method is advantageous to post-elution cleavage for several reasons:

Eliminates most of the impurities normally associated with purification on Ni-chelating columns.

  • Allows much gentler elution conditions, with an added flexibility in the composition of the elution buffer.
  • Assist preventing protein aggregation and inactivation.
  • Following cleavage, the protease can be efficiently removed by using any avidin-conjugated or streptavidin-conjugated beads.

This biotin-tagged SUMO protease has been enzymatically biotinylated without affecting its proteolytic activity. It does not include any additional protein purification tag (e.g., histidine-tag or GST).
Unit Definition【单位定义】
One enzyme unit is defined as the amount that will cut 90% of 100 pmol of SUMO-GST in 1 hour at 30℃.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 2
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