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Sortase A, S. aureus, His-tag
产品编号: | 4140660 |
规格: | Staphylococcus aureus, recombinant, N-terminal His-Tag |
包装规格: | 50 μG |
产品类别: | 进口试剂 |
品牌: | SAFC |
优惠价: | 立即咨询 |
产品别名
Sortase A, S. aureus, His-tag
sortase A
SrtA
产品性质
recombinant【重组】 | expressed in E. coli |
tag【标签】 | His tagged |
form【形式】 | liquid |
mol wt【分子量】 | 27.7 kDa |
UniProt accession no.【UniProt登记号】 | Q9S446 |
storage temp.【储存温度】 | −70℃ |
基本信息
General description【一般描述】 | This product is a purified Staphylococcus aureus Sortase A protein (GenBank Accession No. AF162687), a.a. 26 – 206 (end) with a N-terminal His-tag, expressed in an E. coli expression system. |
Application【应用】 | This product is suitable for in vitro ligation of proteins and peptides to other. Sortase A has been shown to create circularized proteins [1], as well as couple polypeptides to a wide range of substituents including polymers [2], lipids [3], fluorophores [4], sugars [5], microspheres [3], peptide nucleic acids [6], among others. |
Biochem/physiol Actions【生化/生理作用】 | Staphylococcal Sortase A is a bacterial transpeptidase that covalently links proteins to the bacterial cell wall by cleaving between threonine and glycine at an LPXTG recognition motif and catalyzes the formation of an amide bond between the carboxyl-group of threonine and the amino-group of the cell-wall peptidoglycan [7]. This chemistry can be exploited to site-specifically link proteins/peptides with the C-terminal LPETGX motif to other proteins or molecules possessing a glycine or aminomethylene motif [8]. |
Physical form【外形】 | Aqueous buffered solution containing: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 8 mM imidazole, 0.04% Tween-20, and 20% glycerol |
Analysis Note【分析说明】 | Analysis of this product can be performed in a reaction buffer (50 μl) containing 50 mM HEPES (pH=7.4), 150 mM NaCl, 5 mM CaCl2, 5 mM (Gly)3, 25 mM Abz/Dnp substrate, and Sortase A for 30 min at 30℃. Fluorescence intensity is measured at Ex320nm/Em420nm. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |