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Sortase A, S. aureus, His-tag

产品编号:4140660
规格:Staphylococcus aureus, recombinant, N-terminal His-Tag
包装规格:50 μG
产品类别:进口试剂
品牌:SAFC
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
414066050 μG5850
产品别名

Sortase A, S. aureus, His-tag

sortase A

SrtA

产品性质
recombinant【重组】
expressed in E. coli
tag【标签】
His tagged
form【形式】
liquid
mol wt【分子量】
27.7 kDa
UniProt accession no.【UniProt登记号】
Q9S446
storage temp.【储存温度】
−70℃
基本信息
General description【一般描述】
This product is a purified Staphylococcus aureus Sortase A protein (GenBank Accession No. AF162687), a.a. 26 – 206 (end) with a N-terminal His-tag, expressed in an E. coli expression system.
Application【应用】
This product is suitable for in vitro ligation of proteins and peptides to other. Sortase A has been shown to create circularized proteins [1], as well as couple polypeptides to a wide range of substituents including polymers [2], lipids [3], fluorophores [4], sugars [5], microspheres [3], peptide nucleic acids [6], among others.
Biochem/physiol Actions【生化/生理作用】
Staphylococcal Sortase A is a bacterial transpeptidase that covalently links proteins to the bacterial cell wall by cleaving between threonine and glycine at an LPXTG recognition motif and catalyzes the formation of an amide bond between the carboxyl-group of threonine and the amino-group of the cell-wall peptidoglycan [7]. This chemistry can be exploited to site-specifically link proteins/peptides with the C-terminal LPETGX motif to other proteins or molecules possessing a glycine or aminomethylene motif [8].
Physical form【外形】
Aqueous buffered solution containing: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 8 mM imidazole, 0.04% Tween-20, and 20% glycerol
Analysis Note【分析说明】
Analysis of this product can be performed in a reaction buffer (50 μl) containing 50 mM HEPES (pH=7.4), 150 mM NaCl, 5 mM CaCl2, 5 mM (Gly)3, 25 mM Abz/Dnp substrate, and Sortase A for 30 min at 30℃. Fluorescence intensity is measured at Ex320nm/Em420nm.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 2
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