RNA Polymerase II, p33 subunit human

NA.26

hRPB3 (p33) is a highly conserved subunit shared by all three RNA polymerases. It has been shown to be in close contact to promoter DNA when pol II is recruited into the preinitiation complex. RPB3 has also been implicated in direct protein-protein contacts with transcription factor IIB, Rap30 subunit of transcription factor IIF, and gene-specific modulator proteins, such as the hepatitis B virus transactivator protein X or an inhibitor of pol II, RMP (RPB3-mediating protein). Therefore, RPB3 is facilitating the communication between the pol II core and a variety of basal and gene-specific transcription factors. In the pol II complex, RPB3 interacts with RPB5 and the RPB3–RPB5 interaction is intensified in the presence of three subunits, RPB7, RPB8 and RPB11. RPB3 also makes direct contacts with both RPB1 and RPB2, the two large subunits of the polymerase complex.

Clear and colorless frozen liquid solution

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

human

expressed in E. coli

≥80% (SDS-PAGE)

frozen liquid

~33.5 kDa

pkg of 10 μg

avoid repeated freeze/thaw cycles

350 μg/mL

western blot: suitable

clear colorless

NM_032940

P19387

dry ice

−70℃

human ... POLR2C(5432)

10 - Combustible liquids

WGK 1