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RNA Polymerase II, p14.5 subunit human

产品编号:4140388
规格:recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
包装规格:10 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
414038810 μG5700
产品别名

RNA Polymerase II, p14.5 subunit human

hRPB14.5

RPB9

基本信息
NACRES
NA.26
Biochem/physiol Actions【生化/生理作用】
hRPB9 is a subunit unique to RNA polymerase II, although it has sequence homologues in RNA polymerases I and III. The gene for Rpb9 is not essential for yeast cell viability, but is essential in Drosophila. hRPB9 has roles in both transcription initiation and transcription elongation. In the initiation reaction it is necessary for accurate start site selection. In the elongation reaction, RPB9, along with TFIIS facilitates the conversion of an arrest-competent conformation to a read-through competent conformation. RNA polymerase II lacking the RPB9 subunit uses alternate transcription initiation sites in vitro and in vivo and is unable to respond to the transcription elongation factor TFIIS in vitro. A role in the modulation of initiation and elongation is consistent with the localization of RPB9 in the three-dimensional structure of yeast RNA polymerase II. RPB9 is located at the tip of the so-called "jaws" of the enzyme, which is thought to function by clamping the DNA downstream of the active site. RPB9 comprises two zinc ribbon domains joined by a conserved linker region. The C-terminal zinc ribbon is similar in sequence to that found in TFIIS.
Physical form【外形】
Clear and colorless frozen liquid solution
Preparation Note【制备说明】
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
产品性质
biological source【生物来源】
human
recombinant【重组】
expressed in E. coli
Assay【测定】
≥80% (SDS-PAGE)
form【形式】
frozen liquid
mol wt【分子量】
~16.6 kDa
packaging【包装】
pkg of 10 μg
storage condition【储存条件】
avoid repeated freeze/thaw cycles
concentration【浓度】
300 μg/mL
technique(s)
western blot: suitable
color【颜色】
clear colorless
NCBI accession no.【NCBI登记号】
NM_006233
UniProt accession no.【UniProt登记号】
P36954
shipped in【运输】
dry ice
storage temp.【储存温度】
−70℃
Gene Information
human ... POLR2I(5438)
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 1
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