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RNA Polymerase II, p14.5 subunit human
产品编号: | 4140388 |
规格: | recombinant, expressed in E. coli, ≥80% (SDS-PAGE) |
包装规格: | 10 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
RNA Polymerase II, p14.5 subunit human
hRPB14.5
RPB9
基本信息
NACRES | NA.26 |
Biochem/physiol Actions【生化/生理作用】 | hRPB9 is a subunit unique to RNA polymerase II, although it has sequence homologues in RNA polymerases I and III. The gene for Rpb9 is not essential for yeast cell viability, but is essential in Drosophila. hRPB9 has roles in both transcription initiation and transcription elongation. In the initiation reaction it is necessary for accurate start site selection. In the elongation reaction, RPB9, along with TFIIS facilitates the conversion of an arrest-competent conformation to a read-through competent conformation. RNA polymerase II lacking the RPB9 subunit uses alternate transcription initiation sites in vitro and in vivo and is unable to respond to the transcription elongation factor TFIIS in vitro. A role in the modulation of initiation and elongation is consistent with the localization of RPB9 in the three-dimensional structure of yeast RNA polymerase II. RPB9 is located at the tip of the so-called "jaws" of the enzyme, which is thought to function by clamping the DNA downstream of the active site. RPB9 comprises two zinc ribbon domains joined by a conserved linker region. The C-terminal zinc ribbon is similar in sequence to that found in TFIIS. |
Physical form【外形】 | Clear and colorless frozen liquid solution |
Preparation Note【制备说明】 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
产品性质
biological source【生物来源】 | human |
recombinant【重组】 | expressed in E. coli |
Assay【测定】 | ≥80% (SDS-PAGE) |
form【形式】 | frozen liquid |
mol wt【分子量】 | ~16.6 kDa |
packaging【包装】 | pkg of 10 μg |
storage condition【储存条件】 | avoid repeated freeze/thaw cycles |
concentration【浓度】 | 300 μg/mL |
technique(s) | western blot: suitable |
color【颜色】 | clear colorless |
NCBI accession no.【NCBI登记号】 | NM_006233 |
UniProt accession no.【UniProt登记号】 | P36954 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −70℃ |
Gene Information | human ... POLR2I(5438) |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |