您的位置：首页 > 产品中心 > PTP1B (1-321)/PTPN1 Active from rat
PTP1B (1-321)/PTPN1 Active from rat
| 产品编号： | 4140036 |
| 规格： | recombinant, expressed in E. coli, ≥90% (SDS-PAGE) |
| 包装规格： | 20 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
PTP1B (1-321)/PTPN1 Active from rat
Protein tyrosine phosphatase, non-receptor type 1
PTPN1
产品性质
| biological source【生物来源】 | rat |
| recombinant【重组】 | expressed in E. coli |
| Assay【测定】 | ≥90% (SDS-PAGE) |
| form【形式】 | aqueous solution |
| mol wt【分子量】 | 63.3 kDa |
| packaging【包装】 | pkg of 20 μg |
| storage condition【储存条件】 | avoid repeated freeze/thaw cycles |
| concentration【浓度】 | >0.02 mg/mL |
| NCBI accession no.【NCBI登记号】 | NM_012637.2 |
| UniProt accession no.【UniProt登记号】 | P20417 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... PTPN1(5770) |
基本信息
| General description【一般描述】 | Protein tyrosine phosphatase, catalytic domain (amino acids. 1-321), with N-terminal GST tag, MW = 63.3 kDa, expressed in an E. coli expression system. |
| Application【应用】 | Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. |
| Unit Definition【单位定义】 | One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30℃. |
| Physical form【外形】 | Formulation: recombinant proteins were eluted in 25 mM Tris-HCL, 75 mM NaCl, pH 8.0, 0.05% Tween, 10 mM glutathione, 1 mM DTT and 50% glycerol. |
| Preparation Note【制备说明】 | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles. |
