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PI3 kinase [p110a(E545K)/p85a] Active human
| 产品编号： | 4139720 |
| 规格： | recombinant, expressed in baculovirus infected insect cells, ≥55% (SDS-PAGE) |
| 包装规格： | 20 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
PI3 kinase [p110a(E545K)/p85a] Active human
Phosphoinositide-3-kinase, catalytic, alpha
PIK3CA
PtdIns-3-kinase p110
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in baculovirus infected insect cells |
| Assay【测定】 | ≥55% (SDS-PAGE) |
| form【形式】 | aqueous solution |
| Specific Activity | ≥800 pmol/min-μg |
| mol wt【分子量】 | 129 kDa 88 kDa |
| packaging【包装】 | pkg of 20 μg |
| concentration【浓度】 | 0.56 mg/mL |
| NCBI accession no.【NCBI登记号】 | U79143 (p110alpha) XM_043865 (p85alpha) |
| UniProt accession no.【UniProt登记号】 | P42336 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... PIK3CA(5290) |
基本信息
| General description【一般描述】 | Complex of N-terminal FLAG-tagged recombinant full-length human p110 (GenBank Accession No. U79143), with E545K mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system. |
| Application【应用】 | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Unit Definition【单位定义】 | One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37℃. |
| Physical form【外形】 | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT. |
| Preparation Note【制备说明】 | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles. |
