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NEDD4 Active human

产品编号:4139081
规格:recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)
包装规格:20 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
413908120 μG7540
产品别名

NEDD4 Active human

KIAA0093

PIG53

RPF1

neural precursor cell expressed, developmentally down-regulated 4

Cell proliferation-inducing gene 53 protein

基本信息
NACRES
NA.32
General description【一般描述】
Neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4) has an amino terminal domain, four tryptophan-rich WW domains and a carboxy-terminal homologous to the E6-AP carboxyl terminus (HECT) ubiquitin ligase domain. The gene encoding this protein is localized on human chromosome 15q21.3. Human Nedd4 (GenBank Accession No. NM_006154), amino acids 150-end, with N-terminal DDDDK tag (FLAG) MW = 86kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application【应用】
Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions【生化/生理作用】
Neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4) associates with the PPxY (PY) motifs of the subunits of the human epithelial Na+ channel (hENaC). It is a positive and negative modulator of signaling receptors. The protein is overexpressed in endometrial cancer.
Unit Definition【单位定义】
One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37℃.
Physical form【外形】
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.
Preparation Note【制备说明】
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
产品性质
biological source【生物来源】
human
recombinant【重组】
expressed in baculovirus infected insect cells
Assay【测定】
≥60% (SDS-PAGE)
form【形式】
aqueous solution
mol wt【分子量】
86 kDa
packaging【包装】
pkg of 20 μg
concentration【浓度】
>0.02 mg/mL
NCBI accession no.【NCBI登记号】
NM_006154
UniProt accession no.【UniProt登记号】
P46934
shipped in【运输】
dry ice
storage temp.【储存温度】
−70℃
Gene Information
human ... NEDD4(4734)
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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