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NEDD4 Active human
产品编号: | 4139081 |
规格: | recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE) |
包装规格: | 20 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
NEDD4 Active human
KIAA0093
PIG53
RPF1
neural precursor cell expressed, developmentally down-regulated 4
Cell proliferation-inducing gene 53 protein
基本信息
NACRES | NA.32 |
General description【一般描述】 | Neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4) has an amino terminal domain, four tryptophan-rich WW domains and a carboxy-terminal homologous to the E6-AP carboxyl terminus (HECT) ubiquitin ligase domain. The gene encoding this protein is localized on human chromosome 15q21.3. Human Nedd4 (GenBank Accession No. NM_006154), amino acids 150-end, with N-terminal DDDDK tag (FLAG) MW = 86kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
Application【应用】 | Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Biochem/physiol Actions【生化/生理作用】 | Neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4) associates with the PPxY (PY) motifs of the subunits of the human epithelial Na+ channel (hENaC). It is a positive and negative modulator of signaling receptors. The protein is overexpressed in endometrial cancer. |
Unit Definition【单位定义】 | One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37℃. |
Physical form【外形】 | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT. |
Preparation Note【制备说明】 | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles. |
产品性质
biological source【生物来源】 | human |
recombinant【重组】 | expressed in baculovirus infected insect cells |
Assay【测定】 | ≥60% (SDS-PAGE) |
form【形式】 | aqueous solution |
mol wt【分子量】 | 86 kDa |
packaging【包装】 | pkg of 20 μg |
concentration【浓度】 | >0.02 mg/mL |
NCBI accession no.【NCBI登记号】 | NM_006154 |
UniProt accession no.【UniProt登记号】 | P46934 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −70℃ |
Gene Information | human ... NEDD4(4734) |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |