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Monoclonal Anti-ERp57-Atto 488 antibody produced in mouse
产品编号: | 4137870 |
规格: | clone 4F9, affinity isolated antibody, buffered aqueous solution |
包装规格: | 100 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Monoclonal Anti-ERp57-Atto 488 antibody produced in mouse
Anti-PDIA3
基本信息
NACRES | NA.45 |
Immunogen【免疫原】 | Immunogen Region: Other – provide in Additional Product Details Immunogen Description: Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged) Immunogen Species: Human Immunogen type: Recombinant protein |
Application【应用】 | Western blotting Dilution 1:1000 Tissue/Cell Type Used: HeLa cell lysate Immunofluorescence Dilution 1:100 Tissue/Cell Type Used: HeLa |
Physical form【外形】 | 50% glycerol, 0.09% Sodium Azide PBS pH 7.4 |
Preparation Note【制备说明】 | Derived from cell/tissue culture |
Other Notes【其他说明】 | Additional Product Details: Immunogen region: Amino acids 25-505 Lot Expiration/Retest Date: Expires 1 year from purchase Target/Gene Description: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface . ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus . Purification method: Protein G purified Product type: Primary antibody Antibody structure: Whole Antibody Protein ID: NP_005304.3 |
产品性质
biological source【生物来源】 | mouse |
conjugate【偶联物】 | Atto 488 conjugate |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | 4F9 monoclonal |
description【描述】 | clone 4F9, purified immunoglobulin, buffered aqueous solution |
form【形式】 | buffered aqueous solution |
species reactivity | human (Tested) |
packaging【包装】 | pkg of 100 μg |
storage condition【储存条件】 | avoid repeated freeze/thaw cycles protect from light |
concentration【浓度】 | 1.0 mg/mL |
target molecular weight【靶分子量】 | 57 kDa |
technique(s) | immunofluorescence: 1:100 western blot: 1:1000 |
isotype【同位素/亚型】 | IgG2b |
UniProt accession no.【UniProt登记号】 | P30101 |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... PDIA3(2923) |
产品说明
Storage and Stability【储存及稳定性】 | Protect from light; avoid repeated freeze-thaw cycles. Store at -20 ℃ long-term, 2-8 ℃ short-term. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 2 |