Milli-Mark^® Anti-Phosphotyrosine-PE Antibody, recombinant clone 4G10^®

32160702

NA.41

Some of the tyrosine residues can be tagged with a phosphate group by protein kinases and in its phosphorylated state, it is referred to as phosphotyrosine. Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity.
The advent of anti-phosphotyrosine antibodies is one of the significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosine phosphorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyne phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.

Expected to detect tyrosyne phosphorylated proteins in all species.
Antibody recognizes tyrosine-phosphorylated proteins from all species

Milli-Mark Anti-Phosphotyrosine-PE Antibody, recombinant clone 4G10 is an antibody against Phosphotyrosine clone 4G10 for use in FC.

Evaluated by flow cytometry using EGF treated versus untreated A431 cells

Purified mouse monoclonal IgG2bk conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Protein G Purified

Control
Treated versus untreated A431 cells

4G10 is a registered trademark of Upstate Group, Inc.
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

PE

purified immunoglobulin

primary antibodies

4G10, monoclonal

vertebrates, human

Milli-Mark^®

flow cytometry: suitable

IgG2bκ

wet ice

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2