Milli-Mark^® Anti-CD13-FITC Antibody, clone WM-47

32160702

NA.44

CD13 is a 150 kDa Type II integral membrane protein composed of 967 amino acids (Turni, 2002; Ashmun, 1995; Look, 1989). CD13 is identical in structure to aminopeptidase N, a membrane-bound zinc-binding metalloprotease (Turni, 2002). CD13 is expressed on the surface of committed granulocyte-monocyte progenitors (CFU-GM) and by cells of the granulocyte and monocyte lineages at all stages of differentiation (Turni, 2002; Ashmun, 1995; Look, 1989). The antigen is also expressed on a small proportion of large granular lymphocytes, but not on B or T cells (Ashmun, 1995; Look, 1989). Further, CD13 is expressed on some non-hematopoietic cells including fibroblasts, endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining the bile duct canaliculi (Turni, 2002; Ashmun, 1995; Look, 1989).
Anti-CD13, WM-47, was included in the Fourth and Fifth International Workshop and Conference on Leucocyte Differentiation Antigens and studies by a number of laboratories confirmed its reactivity with CD 13 (Ashmun, 1995; Look, 1989).

Antibody recognizes Human CD13 antigen.

Leukaemic cells from a case of chronic myeloid leukaemia (CML).

Detect CD13 using this Milli-Mark Anti-CD13-FITC Antibody, clone WM-47 validated for use in FC.
Research Sub Category
Immunoglobulins & Immunology
Research Category
Inflammation & Immunology

Evaluated by flow cytometry using human PBMCs

Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Protein A purified

Control
PBMCs

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

FITC conjugate

purified immunoglobulin

primary antibodies

WM-47, monoclonal

human

Milli-Mark^®

flow cytometry: suitable

IgG1κ

NP_001141.2

P15144

wet ice

150 kDa (Turni, 2002; Ashmun, 1995; Look, 1989)

Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2