Milli-Mark^® Anti-CD138-APC Antibody, clone MI15

32160702

NA.41

CD138 is a transmembrane proteoglycan, bearing heparin sulphates and sometimes chondriotin sulfates. It consists of a 30.5 kDa core protein with 5 putative glycosaminoglycans attachment sites. CD138 is a member of the syndecan family. The reported Mr of CD138 ranges from 85,000 to 92,000, presumably depending on the glycosaminoglycan pattern of the molecule (Wijdenes, 1997). Within the hematopoietic system, CD138 is mainly confined to late stages of B-cell differentiation. It is typically expressed at high cellular density in plasma cells (Gattei, 1999). In mature tissues, CD138 is expressed by simple and stratified epithelia, fibroblasts, stratified keratinocytes and endothelial cells (Wijdenes, 1997). Anti-CD138, MI15, was included in the Sixth International Workshop and Conference on Human Leucocytes Differentiation Antigens, and studies by a number of different laboratories confirmed its reactivity with CD138 (Wijdenes, 1997).

Antibody recognizes Human CD138 antigen.

A mixture of U266 and XG-1 human myeloma cell lines

Detect CD138-APC using this Milli-Mark Anti-CD138-APC Antibody, clone MI15 validated for use in FC.
Research Sub Category
Immunoglobulins & Immunology
Research Category
Inflammation & Immunology

Evaluated by flow cytometry using U266 cells

Purified Mouse monoclonal IgG1 conjugated to APC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Protein A purified

Control
U266 cells

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

Allophycocyanin conjugate

purified immunoglobulin

primary antibodies

MI15, monoclonal

human

Milli-Mark^®

flow cytometry: suitable

IgG1κ

NP_001006947

P18827

wet ice

85-92 kDa (Wijdenes, 1997)

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2