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DNMT3A/DNMT3L，活性，人
DNMT3A/DNMT3L active human
| 产品编号： | 4117897 |
| 规格： | recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE) |
| 包装规格： | 10 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
DNMT3A/DNMT3L，活性，人
DNMT3A/DNMT3L active human
基本信息
| NACRES | NA.32 |
| General description【一般描述】 | The DNMT3A (DNA (cytosine-5)-methyltransferase 3A) gene is mapped to human chromosome 2p23. The encoded protein has 2 isoforms, DNMT3A1 and DNMT3A2. DNMT3A1 is widely expressed and DNMT3A2 is mainly present in embryonic stem cells (ovaries and testes). DNMT3L (DNA (cytosine-5)-methyltransferase 3-like) belongs to the Dnmt3 family. It is unable to methylate DNA. The protein has a cysteine-rich region containing a novel-type zinc finger domain. The gene is mapped to human chromosome 21q22.3. |
| Application【应用】 | DNMT3A/DNMT3L active human has been used in in vitro methylation assay and REMSA (RNA electrophoretic mobility shift assay) to study role of ecRNAs (extra coding RNAs) in DNA methylation. |
| Biochem/physiol Actions【生化/生理作用】 | DNMT3A (DNA (cytosine-5)-methyltransferase 3A) is a DNA methyltransferase. It is responsible for the methylation of the cytosine residue in 5′-C-phosphate-G-3′ (CpG) dinucleotides. DNMT3A plays a significant role in immunoregulation (adaptive as well as innate immune responses). It is also the most commonly mutated gene in hematologic malignancies. DNMT3A is a commonly mutated gene in acute myeloid leukemia and is responsible for an unfavorable prognosis. DNMT3L (DNA (cytosine-5)-methyltransferase 3-like) is mainly responsible for the stimulation of DNA methylation. It is a non-catalytic accessory factor. It helps the DNA methylation machinery to be placed on properly chromatinized DNA regions. It also helps the poorly methylated area, thereby providing uniform methylation patterns. Mutations in the DNMT3L gene might be associated with azoospermia susceptibility. |
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in baculovirus infected Sf9 cells |
| Assay【测定】 | ≥90% (SDS-PAGE) |
| form【形式】 | aqueous solution |
| mol wt【分子量】 | 34 kDa (MLL1) 52 kDa (DNMT3L) |
| packaging【包装】 | pkg of 10 μg |
| NCBI accession no.【NCBI登记号】 | NM_013369 (DNMT3L) NM_175629 (DNMT3A) |
| UniProt accession no.【UniProt登记号】 | Q9Y6K1 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... DNMT3A(1788), DNMT3L(29947) |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
