Bcl-2, C-Terminal Truncated, His·Tag^®, Human, Recombinant, E. coli

100

human

expressed in E. coli

≥95% (SDS-PAGE)

liquid

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

wet ice

−20℃

50 μg in Glass bottle
Please refer to vial label for lot-specific concentration.

Recombinant, human Bcl-2 without the 17 amino acid mitochondrial targeting sequence at the C-terminus, fused to a His•Tag sequence, and expressed in E. coli. The truncated protein retains the ability to neutralize pro-apoptotic Bcl-2 family members.
Recombinant, human Bcl-2 without the 17 amino acid mitochondrial targeting sequence at the C-terminus, fused to a His-Tag sequence and expressed in E. coli. The truncated protein retains the ability to neutralize pro-apoptotic Bcl-2 family members.

Neutralization (see biological activity)

EC₅₀ = 100-300 nM based on its ability to inhibit 54 nM caspase-8-cleaved human Bid-mediated release of cytochrome c from isolated mouse liver mitochondria

Toxicity: Standard Handling (A)

In 100 mM KCl, 10 mM HEPES, 20% glycerol, pH 8.0.

Following initial thaw, aliquot and freeze (-20°C).

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

10 - Combustible liquids

WGK 1