ATP Citrate Lyase Active human

NA.54

Human ATP citrate lyase (ACLY) (GenBank Accession No. NM_001096), full length, with N-terminal GST tag, MW = 147 kDa, expressed in baculovirus expression system.

Active human ATP citrate lyase is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human ATP citrate lyase has been used in a study to ascertain the nature of the catalytic phosphorylation that initiates the ACL reaction, and to identity the active site residues involved. Active human ATP citrate lyase has also been used in a study to analyze tumor metabolism to reveal mitochondrial glucose oxidation in genetically diverse human glioblastomas.

ATP citrate lyase is an enzyme involved in fatty acid synthesis that generates cytosolic acetyl-CoA and oxaloacetate from citrate and CoA. ATP citrate lyase is often upregulated in cancer.

One unit is defined as the amount of enzyme required to convert 1 pmol of ADP to ATP/min at 37℃.

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

expressed in baculovirus

≥90% (SDS-PAGE)

aqueous solution

≥1.2 pmol/min-μg

147 kDa

NM_001096

dry ice

−70℃

mouse ... ACLY(47)