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aRAF Active human
产品编号: | 4116475 |
规格: | recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE) |
包装规格: | 10 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
aRAF Active human
v-Raf murine sarcoma 3611 viral oncogene homolog
PKS2
A-Raf proto-oncogene
基本信息
NACRES | NA.32 |
General description【一般描述】 | Human A-RAF (GenBank Accession No. NM_001654), amino acids 282-end, containing the mutations YY301-302DD, MW = 63.6 kDa, with N-terminal GST tag, expressed in an Baculovirus infected Sf9 cell expression system. |
Application【应用】 | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Physical form【外形】 | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT. |
Preparation Note【制备说明】 | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles. |
产品性质
biological source【生物来源】 | human |
recombinant【重组】 | expressed in baculovirus infected insect cells |
Assay【测定】 | ≥80% (SDS-PAGE) |
form【形式】 | aqueous solution |
mol wt【分子量】 | 63.6 kDa |
packaging【包装】 | pkg of 10 μg |
storage condition【储存条件】 | avoid repeated freeze/thaw cycles |
concentration【浓度】 | >0.02 mg/mL |
NCBI accession no.【NCBI登记号】 | NM_001654 |
UniProt accession no.【UniProt登记号】 | P10398 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −70℃ |
Gene Information | human ... ARAF(369) |