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AOF ITS Supplement (100X), 100ml | SCM055

产品编号:4116447
规格:AOF ITS supplement is an animal-origin-free product that contains human recombinant insulin, transferrin along with sodium selenite and is used for the serum-free culture of numerous cell types.
包装规格:10 ML,100 ML
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
411644710 ML1220
4116447100 ML7000
产品别名

AOF ITS Supplement (100X), 100ml | SCM055

ITS, Insulin Transferrin Selenite

基本信息
eCl@ss
32160801
NACRES
NA.75
General description【一般描述】
EMD Millipore’s AOF ITS (100X) supplement is an animal-origin-free product that contains human recombinant insulin, human recombinant transferrin along with sodium selenite.

Undefined animal derived media supplements, such as fetal bovine serum, have recently been an area of concern for many cell culturists due to their increased costs, lot to lot variability and increased regulation worldwide. The elimination of fetal bovine serum from cell culture media provides a more defined enviroment to study basic biological questions. Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies such as insulin, transferrin and selenite.

ITS supplements have been proven to replace serum and grow the following cells in serum free or reduced serum conditions: CHO, HEK293, fibroblasts, hybridoma, VERO cells, Hela, HT29, MDCK, Mesenchymal, IPS Cells, Cardiac, kidney, keratinocyte, embryonic, neural stem cell, hepatocytes.
Application【应用】
Research Category
Stem Cell Research
EMD Millipore’s AOF ITS (100X) supplement is an completely animal-origin-free product
Components【组分】
All components are derived using AOF (Animal-Origin-Free) reagents and protocols.

Recombinant Human Insulin is derived from Pichia pastoris (yeast).
Recombinant Human Transferrin is derived from Oryza sativa (rice).
Sodium Selenite is a synthetic chemical.
Quality【质量】
Tested for the ability to support the proliferation of VERO cells in serum free conditions over a 3 day time period.
pH: 7.6-8.0.
Product is sterile.
Endotoxin levels are ≤ 1 EU/mg.
Physical form【外形】
Product is made in an Earle′s Balanced Salt Solution and filtered through a 0.22 micron membrane before vialing. Solution is a light pink clear solution that is free of particulates.
Analysis Note【分析说明】
Control
VERO cells
产品性质
Quality Level【质量水平】
100
form【形式】
liquid
concentration【浓度】
100 ×
technique(s)
cell culture | mammalian: suitable
cell culture | stem cell: suitable
产品说明
Storage and Stability【储存及稳定性】
Store at 2-8°C. Further dilutions should be made into appropriate buffer and/or medium to which protein has been added, such as DMEM/1% Human Serum Albumin or equivalent.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Pictograms【象形图】
GHS07
Signal word【警示用语:】
Warning
Hazard Statements
H302
Precautionary Statements
P264 - P270 - P301 + P312 - P501
Hazard Classifications【危险分类】
Acute Tox. 4 Oral
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 2
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