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Anti-TrpV6 Antibody, clone 4A5.1
产品编号: | 4115624 |
规格: | culture supernatant, clone 4A5.1, from mouse |
包装规格: | 100 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-TrpV6 Antibody, clone 4A5.1
Transient receptor potential cation channel subfamily V member 6, Alu-binding protein with zinc finger domain, Calcium transport protein 1, Calcium transporter-like protein, CaT-L, CaT-like, CaT1, ECaC2, Epithelial apical membrane calcium transporter/cha
基本信息
eCl@ss | 32160702 |
General description【一般描述】 | Transient receptor potential cation channel subfamily V member 6 (UniProt Q9H1D0; also known as Alu-binding protein with zinc finger domain, Calcium transport protein 1, Calcium transporter-like protein, CaT-L, CaT-like, CaT1, ECaC2, Epithelial apical membrane calcium transporter/channel CaT1, Epithelial calcium channel 2, TrpV6) is encoded by the TRPV6 (also known as ABP/ZF, CAT1, CATL, ECAC2, ZFAB) gene (Gene ID 55503) in human. TRPV6 belongs to the transient receptor potential (TRP) family of membrane proteins. The human TRPV6 is an 765-amino acid, six-transmembrane protein with both N- and C-termini exposed intracellularly. TRPV6 contains a pore-forming loop domain located between the fifth and sixth transmembrane region. Among all the TRPV family members, TRPV5 and TRPV6 are the two highly Ca2+-selective channels that play pivotal roles in the Ca2+-reabsorption in the kidney and intestine, respectively. TRPV6 is found in the apical brush-border membrane of the intestinal enterocytes where it regulates calcium entry into the cells. TRPV6 expression is also reported in human placenta, pancreas, and prostate gland. Vitamin D treatment of human colon cancer Caco-2 cells and duodenal explants results in upregulated TRPV6 mRNA levels. |
Specificity【特异性】 | Expected to react with both spliced isoforms of human TrpV6 reported by UniProt (Q9H1D0). |
Immunogen【免疫原】 | Epitope: C-terminal region. GST-tagged recombinant human TrpV6 C-terminal fragment. |
Application【应用】 | Research Category Neuroscience Research Sub Category Developmental Neuroscience Western Blotting Analysis: A 1:500 dilution from a representative lot detected TrpV6 in 10 µg of LNCaP prostate adenocarcinoma and K562 erythroleukemia cell lysates. Immunohistochemistry Analysis: A 1:50-1:250 dilution from a representative lot detected TrpV6 in human pancreas, human small intestine, rat colon tissue, and mouse brain tissues. |
Quality【质量】 | Evaluated by Western Blotting in Y79 retinoblastoma cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected TrpV6 in 10 µg of Y79 retinoblastoma cell lysate. |
Physical form【外形】 | Unpurified In culture supernatant without preservatives. |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | culture supernatant |
antibody product type | primary antibodies |
clone【克隆】 | 4A5.1, monoclonal |
species reactivity | human, mouse, rat |
technique(s) | immunohistochemistry: suitable western blot: suitable |
isotype【同位素/亚型】 | IgG2aκ |
NCBI accession no.【NCBI登记号】 | NP_061116 |
UniProt accession no.【UniProt登记号】 | Q9H1D0 |
shipped in【运输】 | dry ice |
产品说明
Target description【目标描述】 | ~70 kDa observed. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 2 |