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Anti-Tropomyosin Antibody, clone 15D12.2
产品别名
Anti-Tropomyosin Antibody, clone 15D12.2
tropomyosin 1 (alpha), cardiomyopathy, hypertrophic 3, tropomyosin 1 alpha chain, HTM-alpha, Alpha-tropomyosin, chromosome 15 open reading frame 13, Tropomyosin-1, sarcomeric tropomyosin kappa, alpha tropomyosin
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Tropomyosins (Tms) are a family of >40 highly conserved protein isoforms resulting from alternative splicing of four different genes during development and that are expressed in numerous cell types. These proteins form coiled-coil heterodimers which bind to actin polymers along the α-helical grove. Tropomyosin’s function varies depending on cell type in which it is expressed. Within muscle, Tms play a role in the regulation of actin-myosin interplay for the purpose of mediating muscle contraction. In non-muscle cells, studies suggest a role in the stabilization of actin filaments, along with protecting actin filaments from the severing action of gelsolin and ADF/cofilin depolymerization. Tms also appear to have a role in the moderation of access to actin for other actin-binding proteins. Tropomyosin has an integral role in maintaining cardiovascular homeostasis. Down-regulation of Tms expression has been observed in tumor cells indicating a possible role for Tms as tumor suppressors. |
Specificity【特异性】 | The antibody recognizes the entire exon 9d of Tropomyosin. |
Immunogen【免疫原】 | Linear peptide corresponding to the entire exon 9d of rat Tropomyosin. Epitope: Exon 9d |
Application【应用】 | Research Sub Category Cytoskeleton Muscle Physiology Research Category Cell Structure Metabolism Detect Tropomyosin using this Anti-Tropomyosin Antibody, clone 15D12.2 validated for use in WB, IH. Immunohistochemistry Analysis: 1:300 dilution from a representative lot detected Tropomyosin in human striated muscle tissue. |
Quality【质量】 | Evaluated by Western Blot in mouse brain tissue lysate. Western Blot Analysis: 0.5 µg/mL of the antibody detected Tropomyosin in 10 µg of mouse brain tissue lysate. |
Physical form【外形】 | Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide. Format: Purified Protein G |
Analysis Note【分析说明】 | Control Mouse brain tissue lysate |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | 15D12.2, monoclonal |
species reactivity | human, mouse |
species reactivity (predicted by homology) | rat (based on 100% sequence homology) |
technique(s) | immunohistochemistry: suitable western blot: suitable |
isotype【同位素/亚型】 | IgG2bκ |
NCBI accession no.【NCBI登记号】 | NP_001029243 |
UniProt accession no.【UniProt登记号】 | Q923Z2 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | ~ 30-40 kDa observed MWs. Tm6: 40 kDa; Tm1: 38 kDa; Tm2: 36 kDa; Tm3: 34 kDa; Tm5: 30 kDa |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |