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Anti-Tristetraprolin Antibody
产品编号: | 4115581 |
规格: | from rabbit, purified by affinity chromatography |
包装规格: | 100 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-Tristetraprolin Antibody
Tristetraprolin, TTP, G0/G1 switch regulatory protein 24, Growth factor-inducible nuclear protein NUP475, Protein TIS11A, TIS11, Zinc finger protein 36 homolog, Zfp-36
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Tristetraprolin (TTP, Nup 475, TIS11, G0S24, or ZFP36) is a member of the CCCH tandem zinc finger protein family. It is a nuclear RNA-binding protein that functions as a trans-acting factor. It is involved in post-transcriptional regulation of cytokine genes, most notably Tumor Necrosis Factor alpha (TNF-α). Upon activation by lipopolysaccharides, TTP binds TNF-α mRNA at AU-rich domains in the 3′-untranslated Region (3′-UTR) and induces deadenylation, thereby destabilizing the mRNA and tagging it for degradation. To produce this effect, TTP recruits other trans-acting factors to its C-terminal domain and mRNA-decay enzymes to its N-terminal domain. TTP is deactivated by phosphorylation driven by mitogen-activated protein kinase-activated protein kinase 2 (MK2) and p53. Therefore, TTP/MK2 regulates the biosynthesis of TNF-α and mediates inflammatory responses involving TNF-α. Knockout of TTP in mice produces arthritis, alopecia, dermatitis, among a range of other phenotypes related to overexpression of TNF-α. |
Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to human Tristetraprolin. |
Application【应用】 | Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to detect Tristetraprolin in human leukocytes. (Brooks, S., et al. (2002). Arthritis and Rheumatism. 46(5):1362–1370.) Research Sub Category Growth Factors & Receptors Research Category Epigenetics & Nuclear Function Anti-Tristetraprolin Antibody is an antibody against Tristetraprolin for use in Western Blotting, ICC, Immunoprecipitation, RNA Binding Protein Immunoprecipitation (RIP). |
Quality【质量】 | Evaluated by Western Blot in A431 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected Tristetraprolin on 10 µg of A431 cell lysate. |
Physical form【外形】 | Purified rabbit polyclonal in buffer containing PBS without 0.05% sodium azide. Affinity purified |
Analysis Note【分析说明】 | Control A431 cell lysate |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | human, mouse |
technique(s) | RIP: suitable immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NP_003398 |
UniProt accession no.【UniProt登记号】 | P26651 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | ~40 kDa observed. Uniprot gives a calculated molecular weight of 34 kDa An uncharacterized band appears at ~53 kDa in some lysates. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |