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Anti-TRAP-1 (HSP75)
| 产品编号： | 4115491 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-TRAP-1 (HSP75)
Heat shock protein 75 kDa mitochondrial, TNFR-associated protein 1, Tumor necrosis factor type 1 receptor-associated protein, TRAP-1
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Heat shock protein 75 kDa, mitochondrial (UniProt: Q12931; also known as HSP 75, TNFR-associated protein 1, Tumor necrosis factor type 1 receptor-associated protein, TRAP-1) is encoded by the Trap1 (also known as Hsp75) gene (Gene ID: 287069) in human. TRAP-1 is a mitochondrial protein that serves as a chaperone with ATPase activity. Its ATPase activity is inhibited by small molecule pocket antagonists, such as geldanamycin or radicicol. TRAP-1 is expressed in skeletal muscle, liver, heart, brain, kidney, pancreas, lung, placenta and bladder. Higher expression levels are observed in several cancer cell lines. However, bladder and renal cancers display reduced levels of TRAP-1. TRAP-1 is involved in maintaining mitochondrial function and polarization, likely through stabilization of mitochondrial complex I. Two isoforms of TRAP-1 have been reported that are produced by alternative splicing. It is synthesized with a transit peptide (aa 1-50) that is subsequently cleaved off. TRAP-1 is reported to protect cells against mitochondrial apoptosis and helps cells to cope with oxidative stress. (Ref.: Altieri, DC et al. (2012). Biochim. Biophys. Acta 1823(3); 767-773). |
| Specificity【特异性】 | This rabbit polyclonal antibody detects Tumor necrosis factor type 1 receptor-associated protein (TRAP-1; HSP75) in human cells. It targets an epitope within 12 amino acids from the C-terminal half. |
| Immunogen【免疫原】 | KLH-conjugated linenar peptide corresponding to 12 amino acids from the C-terminal half of human TRAP-1 (HSP75). The immunogen sequence is conserved in both isoforms. |
| Application【应用】 | Western Blotting Analysis: 2 µg/mL from a representative lot detected TRAP-1 (HSP75) in 10 µg of HeLa cell lysate. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected TRAP-1 (HSP75) in human brain, human tonsil, and human prostate cancer tissues. Anti-TRAP-1 (HSP75), Cat. No. ABS1489, is a rabbit polyclonal antibody that detects TRAP-1 (HSP75) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Quality【质量】 | Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected TRAP-1 (HSP75) in 10 µg of HepG2 cell lysate. |
| Other Notes【其他说明】 | Affinity Purified |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology), mouse (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_057376.2 |
| UniProt accession no.【UniProt登记号】 | Q12931 |
| shipped in【运输】 | ambient |
产品说明
| Target description【目标描述】 | ~75 kDa observed; 80.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| 【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
