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Anti-TFE3 Antibody
| 产品编号： | 4115151 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-TFE3 Antibody
TFE3, Transcription factor E3, Class E basic helix-loop-helix protein 33, bHLHe33
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | The DNA-binding factor TFE3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. TFE3 is ubiquitously expressed and can directly associates with DNA as either homodimers or heterodimers formed with two related proteins, TFEB or TFEC. TFE3 binds to and activates the microE3 motif of the immunoglobulin heavy-chain enhancer to induce B-cell-specific gene transcription and DNA recombination. TFEB is expressed at low levels in the embryo but at high levels in the trophoblast cells of the placenta, where it plays a critical role in regulating normal vascularization of the placenta. Recently, Smith et al. have proven that the exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor TFE3 (Cell 153, 335–347, April 11, 2013). |
| Immunogen【免疫原】 | linear peptide corresponding to Human TFE3. |
| Application【应用】 | This Anti-TFE3 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of TFE3. Immunocytochemistry Analysis: A representative lot was used by an independent laboratory on TFE3 overexpressing cell lines, epiblast and primitive endodermal cells (Smith, A., et al. (2013). Cell. 153, 335–347). Research Sub Category Pluripotent & Early Differentiation Research Category Stem Cell Research |
| Quality【质量】 | Evaluated by Western Blot in a HEK293T cell lysate. Western Blot Analysis: 2 µg/mL of this antibody detected TFE3 in 10 µg of HEK293T cell lysate. Uncharacterized band(s) may appear in some lysates with longer exposure times. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing PBS, 0.05% sodium azide. Antigen Affinity Purified |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_006512 |
| UniProt accession no.【UniProt登记号】 | P19532 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... TFE3(7030) |
产品说明
| Target description【目标描述】 | ~ 65 kDa observed |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 2 |
