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Anti-PI3 Kinase p85 Antibody, N-SH2 domain
产品编号: | 4112775 |
规格: | from rabbit, purified by affinity chromatography |
包装规格: | 100 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-PI3 Kinase p85 Antibody, N-SH2 domain
Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-al
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Phosphatidylinositol 3-kinase regulatory subunit alpha (UniProt Q63787; also known as Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha) is encoded by the Pik3r1 gene (Gene ID 25513) in rat species. PI3K regulatory subunit alpha catalyzes the phosphorylation of PI(4,5)P2 inositol ring at the 3 position to generate PI(3,4,5)P3, a potent second messenger that mediates survival signaling and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Following ligand binding, tyrosine phosphorylation of growth factor receptors creates docking sites for p85 binding (through its SH2 domains), and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Plays a role in insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Mutations in Pik3r1 gene are known to be associated with insulin resistance. |
Specificity【特异性】 | This polyclonal antibody targets the first SH2 domain region common to human (isoform 1, AS53, p46, p85I), mouse, and rat (isoform p85-alpha, p55-alpha, and p50-alpha) spliced isoforms of PI3 kinase regulatory subunit alpha reported by UniProt (P27986, P26450, Q63787). |
Immunogen【免疫原】 | GST-tagged recombinant fragment corresponding to the first SH2 domain of rat PI3 kinase regulatory subunit alpha. Epitope: First SH2 domain. |
Application【应用】 | Research Category Signaling Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated PI3 kinase regulatory subunit alpha isoforms from 200 µg of rat brain tissue lysate. Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated PI3 kinase regulatory subunit alpha isoforms from 500 µg of Jurkat cell lysate. Western Blotting Analysis: 2 µg/mL from a representative lot detected PI3 kinase regulatory subunit alpha isoforms in 10 µg of Jurkat cell lysate. Detect PI3 Kinase regulatory subunit alpha using this rabbit polyclonal Anti-PI3 Kinase p85, N-SH2 domain, Cat. No. ABS233, validated for use in Immunoprecipitation and Western Blotting. Research Sub Category Developmental Signaling |
Quality【质量】 | Evaluated by Western Blotting in rat brain tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected PI3 kinase regulatory subunit alpha isoforms in 10 µg of rat brain tissue lysate. |
Linkage【联系】 | Replaces: 06-496 |
Physical form【外形】 | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
Analysis Note【分析说明】 | Control Jurkat cell lysate |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | human, rat |
species reactivity (predicted by homology) | bovine (based on 100% sequence homology), monkey (based on 100% sequence homology), mouse (based on 100% sequence homology) |
technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NP_037137 |
UniProt accession no.【UniProt登记号】 | Q63787 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | ~85/50 kDa observed. 83.47/53.49/49.97/84.47 kDa (human isoform 1/AS53/p46/p85I; UniProt P27986), 83.39 kDa (mouse; UniProt P26450), 83.40/53.39/50.00 kDa (rat p85-alpha/p55-alpha/p50-alpha isoforms; UniProt Q63787). Uncharacterized bands may be observed in some lysate(s). |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |