Anti-phospho SIRT1 (Ser682) Antibody

32160702

NA.41

NAD-dependent protein deacetylase sirtuin-1 (UniProt Q96EB6; also known as hSIR2, hSIRT1, NAD-dependent deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, Sir2-like 1, SIR2-like protein 1, SIR2alpha, Sirtuin type 1) is encoded by the SIRT1 (also known as SIR2L1) gene (Gene ID 23411) in human. Sirtuins constitute a family of NAD+-dependent deacetylases found in nearly all organisms studied. Sirtuins have been implicated in the regulation of multiple processes, including aging, transcription, apoptosis, and stress resistance. Full-length SirT1 (FLSirT1) plays an important role in human cartilage homeostasis and chondrocyte survival by mediating the expression of major cartilage anabolic components, collagen 2(I) and aggrecan. The generation of the transcriptionally inactive 75-kDa fragment (75SirT1) via site-specific cleavage of FLSirT1 at amino acid 533 by cathepsin B is an anti-apoptotic mechanism to promote human osteoarthritic (OA) chondrocytes survival following exposure to proinflammatory cytokines. The 5SirT1 fragment is shown to associate with cytochrome c and likely blocks downstream apoptosome assembly. Phosphorylation plays an important role in modulating SirT1 activity. Multiple kinases and their corresponding SirT1 phosphorylation sites have been identified, including pT344 targeted by AMPK, pT530 and pS540 targeted by Cdk1/Cyclin B, pS46 (mouse) or pS47 (human) targeted by JNK1, pT522 targeted by DYRK1A & DYRK3, as well as pS27 and pS47 targeted by CaMKKβ.

Ser682 of hSIRT1 isoform 1 is equivalent to Ser496 of hSIRT1 spliced isoform 2 (delta-exon8).

KLH-conjugated linear peptide corresponding to human phospho SIRT1 (Ser682) near the C-terminus.

Anti-phospho SIRT1 (Ser682) Antibody, Cat. No. ABE1392, is a highly specific rabbit Polyclonal antibody, that targets SIRT1 and has been tested in Western Blotting.

Evaluated by Western Blotting in Nocodazole treated H1299 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected phospho SIRT1 (Ser682) in 10 µg of Nocodazole treated cell lysate.

Affinity purified

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human

mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), feline (based on 100% sequence homology), porcine (based on 100% sequence homology)

western blot: suitable

NP_036370

Q96EB6

dry ice

human ... SIRT1(23411)

~110 kDa observed. The target band size appears larger than the calculated molecular weight (81.7 kDa/isoform 1 & 61.1 kDa/isoform 2). Uncharacterized band(s) may appear in some lysates.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1