Anti-OSTM1

32160702

NA.41

Osteopetrosis-associated transmembrane protein 1 (UniProt: Q86WC4; also known as Chloride channel 7 beta subunit) is encoded by the OSTM1 (also known as GL) gene (Gene ID: 28962) in human. Osteopetrosis-associated transmembrane protein 1 is a single-pass type I membrane protein that is required for osteoclast and melanocyte maturation and function. It is synthesized with a signal peptide of 31 amino acids, which is cleaved to produce the mature protein. Loss of OSTM1 leads to the most severe form of osteoporosis in mice and human. Although its role in bone chemistry is well established, several studies have shown its expression in neurons, microglia, and astrocytes. OSTM1-deficiecy in the central nervous system leads to gliosis with increased astrocyte cell population and microglia activation along with retinal degeneration at 2 to 3 weeks of age in mice. (Ref.: Ref.: Heraud, C., et al. 2014. J. Biol. Chem. 289(20); 13912-25).

This rabbit polyclonal antibody detects Osteopetrosis-associated transmembrane protein 1 in human cells. It targets an epitope within 18 amino acids from the C-terminus region.

KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminus region of human Osteopetrosis-associated transmembrane protein 1 (OSTM1).

Epitope: C-terminus

Anti-OSTM1, Cat. No. ABN1365, is a highly specific rabbit polyclonal antibody that targets Osteopetrosis-associated transmembrane protein 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Neuroscience
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected OSTM1 in human pancreas and human liver tissues.

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected OSTM1 in 20 µg of human liver tissue lysate.

Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH 7.4 with 0.05% sodium azide.

Concentration: Please refer to lot specific datasheet.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human

bovine (based on 100% sequence homology), baboon (based on 100% sequence homology)

antibody small pack of 25 μg

immunohistochemistry: suitable (paraffin)
western blot: suitable

NP_054747.2

Q86WC4

ambient

~37 kDa observed; 37.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1