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Anti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1
产品别名
Anti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1
Myelin A1 protein, Myelin membrane encephalitogenic protein, myelin basic protein
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | The classic group of MBP isoforms (isoforms 4-14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoforms 1-3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. Isoform 1 Golli-MBP1, HOG7, 33 kDa Isoform 2 Golli-MBP2, HOG5, 21.5 kDa Isoform 3 MBP1, 21.5 kDa Isoform 4 MBP2, 20.2 kDa Isoform 5 MBP3, 18.5 kDa Isoform 6 MBP4, 17.2 kDa (SP_P02686) |
Specificity【特异性】 | Reacts with MBP from human, bovine and rat, epitope 129-138 |
Immunogen【免疫原】 | Bovine myelin basic protein Epitope: a.a. 129-138 |
Application【应用】 | Research Sub Category Neuronal & Glial Markers Neurochemistry & Neurotrophins This Anti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1 is validated for use in ELISA, IH, IH(P), RIA, WB for the detection of Myelin Basic Protein. Research Category Neuroscience Immunohistochemistry(paraffin) Analysis: Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum Immunohistology on frozen sections at 1:10 Western Blot Analysis: A previous lot of this antibody was used in Western Blot. ELISA: A 1:200-1:1,000 dilution of a previous lot was used in ELISA. RIA: A previous lot of this antibody was used in Radioimmunoassay. Optimal working dilutions must be determined by end user. |
Quality【质量】 | Routinely evaluated by immunohistochemistry on brain tissue. Immunohistochemistry(paraffin) Analysis: MBP (cat. # MAB382) staining pattern/morphology in rat cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:50, using IHC-Select Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum |
Physical form【外形】 | Culture supernatant containing 0.2 M Tris/HCl, pH 7.4 with 5-10% fetal calf serum and 0.1% sodium azide Unpurified |
Analysis Note【分析说明】 | Control Brain tissue |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | culture supernatant |
antibody product type | primary antibodies |
clone【克隆】 | 1, monoclonal |
species reactivity | rat, human, rabbit (weakly), bovine |
should not react with | guinea pig |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable radioimmunoassay: suitable western blot: suitable |
isotype【同位素/亚型】 | IgG2a |
NCBI accession no.【NCBI登记号】 | NM_001025094.1 |
UniProt accession no.【UniProt登记号】 | P02686 |
shipped in【运输】 | dry ice |
产品说明
Target description【目标描述】 | 19 kDa |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |